Department of Food and Drug, ShenZhen Polytechnic University, Liuxian Avenue, Nanshan District, Shenzhen, Guangdong, 518055, China.
Department of Food and Drug, ShenZhen Polytechnic University, Liuxian Avenue, Nanshan District, Shenzhen, Guangdong, 518055, China.
Anal Biochem. 2024 Sep;692:115559. doi: 10.1016/j.ab.2024.115559. Epub 2024 May 8.
Bacteremia, as a serious infectious disease, has an increasing incidence and a high mortality rate. Early diagnosis and early treatment are crucial for improving the cure rate. In this work, we proposed an inductively coupled plasma mass spectrometry (ICP-MS)-based detection method combined with gold nanoparticle (Au NP) and silver nanoparticle (Ag NP) labeling for the simultaneous detection of Salmonella and Escherichia coli (E. coli O157:H7) in human blood samples. Salmonella and E. coli O157:H7 were captured by magnetic beads coupled with anti-8G3 and anti-7C2, and then specifically labeled by Au NP-anti-5H12 and Ag NP-anti-8B1 respectively, which were used as signal probes for ICP-MS detection. Under the optimal experimental conditions, the limits of detection of 164 CFU mL for Salmonella, 220 CFU mLfor E. coli O157:H7 and the linear ranges of 400-80,000 CFU mLSalmonella, 400-60,000 CFU mL E. coli O157:H7 were obtained. The proposed method can realize the simultaneous detection of two types of pathogenic bacteria in human whole blood in 3.5 h, showing great potential for the rapid diagnosis of bacteremia in clinic.
菌血症作为一种严重的传染病，发病率不断上升，死亡率也很高。早期诊断和早期治疗对于提高治愈率至关重要。在这项工作中，我们提出了一种基于电感耦合等离子体质谱（ICP-MS）的检测方法，结合了金纳米粒子（AuNP）和银纳米粒子（AgNP）标记，用于同时检测人血样中的沙门氏菌和大肠杆菌（E. coli O157：H7）。沙门氏菌和 E. coli O157：H7 被与抗 8G3 和抗 7C2 偶联的磁珠捕获，然后分别用 AuNP-抗 5H12 和 AgNP-抗 8B1 特异性标记，作为 ICP-MS 检测的信号探针。在最佳实验条件下，沙门氏菌的检测限为 164 CFU mL，E. coli O157：H7 的检测限为 220 CFU mL，沙门氏菌的线性范围为 400-80,000 CFU mL，E. coli O157：H7 的线性范围为 400-60,000 CFU mL。该方法可以在 3.5 小时内实现人全血中两种致病菌的同时检测，为临床菌血症的快速诊断提供了很大的潜力。
