Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Oral and Dental Disease Research Center, Shiraz Dental School, Shiraz University of Medical Sciences, Shiraz, Iran.
Eur Arch Paediatr Dent. 2024 Jun;25(3):443-450. doi: 10.1007/s40368-024-00905-7. Epub 2024 May 9.
This study aimed to evaluate the antibacterial and cytotoxic effects of reinforced zinc oxide-eugenol (rZOE) incorporated with different concentrations of silver nanoparticles (AgNPs).
The pastes of rZOE alone or mixed with AgNPs at concentrations of 1%, 2%, and 5% of weight were prepared. In vitro antimicrobial activity of prepared materials against Streptococcus (S.) mutans and Lactobacillus (L.) acidophilus were evaluated after 2, 4, and 6 h of contact times using direct contact test (DCT) and also following 24 h incubation by well-diffusion test (WDT). The cytotoxicity of the tested materials on human dental pulp stem cells was also determined by MTT assay.
The DCT demonstrated that the time-dependent reductions of the colony numbers of both bacteria by three different concentrations of AgNPs incorporated into rZOE were equal but steeper than the rZOE alone (P < 0.05). The increases in growth inhibition zones of S. mutans and L. acidophilus were associated with the increasing concentration of AgNPs mixed with rZOE in the WDT; however, statistical analysis did not show any significant differences (P = 0.092). The MTT assay revealed a significantly lower percentage of cell viability after 1 day of culture only with the rZOE + AgNP5% in comparison to the rZOE alone (P = 0.011) and the control medium (P = 0.001).
Since the antimicrobial activities of three different concentrations of AgNPs incorporated into rZOE were equal and AgNPs had lower toxicity at lower concentrations, using AgNPs at 1% concentration is suggested to be mixed with rZOE.
本研究旨在评估强化氧化锌丁香酚（rZOE）中添加不同浓度的银纳米粒子（AgNPs）的抗菌和细胞毒性作用。
制备 rZOE 单独或与浓度为 1%、2%和 5%（重量比）的 AgNPs 混合的糊剂。使用直接接触试验（DCT）评估接触 2、4 和 6 小时后制备材料对变形链球菌（S. mutans）和嗜酸乳杆菌（L. acidophilus）的体外抗菌活性，同时通过平板扩散试验（WDT）在 24 小时孵育后进行评估。通过 MTT 测定还确定了测试材料对人牙髓干细胞的细胞毒性。
DCT 表明，三种不同浓度的 AgNPs 掺入 rZOE 后，细菌菌落数量随时间呈依赖性减少，但比单独的 rZOE 减少更陡峭（P < 0.05）。在 WDT 中，随着 rZOE 中 AgNPs 浓度的增加，S. mutans 和 L. acidophilus 的抑菌圈直径增大；然而，统计学分析显示没有显著差异（P = 0.092）。MTT 试验显示，与单独的 rZOE（P = 0.011）和对照培养基（P = 0.001）相比，只有 rZOE + AgNP5%在培养 1 天后细胞活力的百分比显著降低。
由于三种不同浓度的 AgNPs 掺入 rZOE 的抗菌活性相同，并且 AgNPs 在较低浓度下毒性较低，因此建议将 AgNPs 以 1%的浓度与 rZOE 混合使用。
