Beaujean F, Gourdin M F, Farcet J P, Le Forestier C, Bouguet J, Reyes F, Duedari N
Transfusion. 1985 Mar-Apr;25(2):152-4. doi: 10.1046/j.1537-2995.1985.25285169210.x.
A blood processor (IBM 2991) was used to separate lymphocytes from large volumes of blood. The procedure included the centrifugation of 200 ml whole blood on a density gradient. The results of this procedure were compared with those obtained with a manual procedure. Mononuclear cell (MNC) viability was preserved well in the two methods. But with the processor, recovery of MNC was better (63.5 +/- 2.5%) than with manual separation (26.5 +/- 4.1%). Monoclonal antibodies were used to identify the various cell subsets in the MNC fractions. No particular cell selection was observed when MNC fractions were obtained by the separator. In conclusion, the use of a cell separator provided an efficient technique for rapid isolation of large quantities of lymphocytes.
使用血液处理器（IBM 2991）从大量血液中分离淋巴细胞。该程序包括在密度梯度上对200毫升全血进行离心。将该程序的结果与手动程序获得的结果进行比较。两种方法中单核细胞（MNC）的活力均得到良好保存。但是使用该处理器时，MNC的回收率（63.5 +/- 2.5%）比手动分离（26.5 +/- 4.1%）更好。使用单克隆抗体鉴定MNC组分中的各种细胞亚群。当通过分离器获得MNC组分时，未观察到特定的细胞选择。总之，使用细胞分离器提供了一种快速分离大量淋巴细胞的有效技术。
