Sun Chun-Hong, Yao Jian-Na, Liu Fang, Wang Xiao-Juan, Ma Juan-Juan, Cui Mi-Mi, Zhang Mei, Wang Xiao-Ning
Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Dec;24(6):1869-1872. doi: 10.7534/j.issn.1009-2137.2016.06.046.
To investigate the effects of different hemapheresis procedures on the components of hematopoietic stem cells(HSCs) collected from helathy donors.
twelve donors were underwent stem cell collection from January 2015 to August 2016, and the stem cells were randomly colleted by AutoPBSC procedure of COBE spectra and MNC procedure of the Spectra Optia blood cell separator, the mononuclear cells, CD34 cells, granulocytes, lymphocytes and platelets in the collections were compared.
The circulating blood volume, the acquisition time and dosage of anticoagulants were not significantly different between two procedures. The volume and the mononuclear cell count collected by AutoPBSC procedure were lower than those by the MNC procedure, while the CD34 cell count by AutoPBSC procedure was higher than that by the MNC procedure. More lymphocytes and platelets were collected by AutoPBSC procedure as compared with that by the MNC procedure (P<0.05).
Compared with MNC procedure of the Spectra Optia blood cell separator, the number of collected stem cells, lymphocytes and platelets are higher by using AutoPBSC procedure of the COBE spectra blood cell separator.
探讨不同血液成分单采程序对从健康供者采集的造血干细胞（HSCs）成分的影响。
2015年1月至2016年8月对12名供者进行干细胞采集，采用COBE spectra的自动外周血干细胞采集程序（AutoPBSC）和Spectra Optia血细胞分离机的单核细胞采集程序（MNC）随机采集干细胞，比较采集中的单核细胞、CD34细胞、粒细胞、淋巴细胞和血小板。
两种程序之间的循环血量、抗凝剂的采集时间和用量无显著差异。AutoPBSC程序采集的体积和单核细胞计数低于MNC程序，而AutoPBSC程序的CD34细胞计数高于MNC程序。与MNC程序相比，AutoPBSC程序采集的淋巴细胞和血小板更多（P<0.05）。
与Spectra Optia血细胞分离机的MNC程序相比，使用COBE spectra血细胞分离机的AutoPBSC程序采集的干细胞、淋巴细胞和血小板数量更多。
