生物材料孵育后的胰岛移植对链脲佐菌素诱导糖尿病大鼠糖尿病肾病的改善作用。

Improvement effects of transplanting pancreatic islet that previously incubated with biomaterials on the diabetic nephropathy in STZ- diabetic rats.

机构信息

Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

School of Dentistry, Shiraz University of Medical Science, Shiraz, Iran.

出版信息

BMC Nephrol. 2024 May 9;25(1):156. doi: 10.1186/s12882-024-03572-4.

DOI:10.1186/s12882-024-03572-4

PMID:38724923

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11080158/

Abstract

BACKGROUND

Islet transplantation is an effective treatment for diabetes or even its complications. Aim of this study is to investigate efficacy of biomaterial treated islet transplantation on treating diabetic nephropathy.

METHODS

Male rats were randomly divided into 6 groups; Control, diabetic control, diabetic transplanted with untreated islets, with platelet rich plasma treated islets, with pancreatic islets homogenate treated islets, or with these biomaterials combination treated islets. Islets cultured with biomaterials and transplanted to diabetic rats. After 60 days, biochemical, oxidative stress, and stereological parameters were assessed.

RESULTS

Serum albumin and BUN concentration, decreased and increased respectively, Oxidative stress of kidney impaired, kidney weight, volume of kidney, cortex, medulla, glomerulus, proximal and distal tubules, collecting ducts, vessels, inflammatory, necrotic and fibrotic tissue in diabetic group increased compared to control group (p < 0.001). In treated groups, especially pancreatic islets homogenate treated islets transplanting animals, there was significant changes in kidney weight, and volume of kidney, proximal and distal tubules, Henle's loop and collecting ducts compared with diabetic group (p = 0.013 to p < 0.001). Combination treated islets animals showed significant increase in vessel volume compared to diabetic group (p < 0.001). Necrotic and fibrotic tissue significantly decreased in islets treated than untreated islet animals, it was higher in pancreatic islets homogenate, and combination treated islets groups (p = 0.001).

CONCLUSIONS

Biomaterials treated islets transplanting could improve diabetic nephropathy. Improvement of oxidative stress followed by controlling glucose level, and effects of growth factors presenting in biomaterials can be considered as capable underlying mechanism of ameliorating inflammatory, necrotic and fibrotic tissue volume.

摘要

背景

胰岛移植是治疗糖尿病甚至其并发症的有效方法。本研究旨在探讨生物材料处理的胰岛移植治疗糖尿病肾病的疗效。

方法

雄性大鼠随机分为 6 组；对照组、糖尿病对照组、未处理胰岛移植组、富含血小板血浆处理胰岛移植组、胰岛匀浆处理胰岛移植组、以及这些生物材料组合处理胰岛移植组。将胰岛与生物材料共培养后移植至糖尿病大鼠。60 天后，评估生化、氧化应激和体视学参数。

结果

血清白蛋白和 BUN 浓度降低，氧化应激损伤的肾脏、肾脏重量、肾脏体积、皮质、髓质、肾小球、近端和远端肾小管、收集管、血管、炎症、坏死和纤维化组织增加，与对照组相比，糖尿病组（p<0.001）。在治疗组中，特别是胰岛匀浆处理胰岛移植动物，与糖尿病组相比，肾脏重量、肾脏体积、近端和远端肾小管、亨利氏环和收集管均有显著变化（p=0.013 至 p<0.001）。组合处理胰岛动物与糖尿病组相比，血管体积显著增加（p<0.001）。与未处理胰岛动物相比，生物材料处理的胰岛动物的坏死和纤维化组织显著减少，在胰岛匀浆和组合处理胰岛组中更高（p=0.001）。