Mahmoudzadeh-Sagheb H, Heidari Z, Bokaeian M, Moudi B
Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Folia Morphol (Warsz). 2010 May;69(2):112-8.
The leaves of Eucalyptus globulus (eucalyptus) are used for the treatment of diabetes mellitus in traditional medicine. The aim of this study was to evaluate the effects of eucalyptus on streptozotocin (STZ)-induced damage in pancreatic islands by stereological methods. Fifty mature normoglycaemic male Wistar rats, weighing 200-250 g, were selected and randomly divided into 5 groups (n = 10): control; STZ-induced diabetic (D) - by intraperitoneal injection of 60 mg/kg streptozotocin; treated control (TC); and treated diabetic (TD1, 2), respectively, received 20 and 62.5 g/kg of eucalyptus in their diet, and 2.5 g/L aqueous extract of eucalyptus in their drinking water from one week after induction of diabetes. After four weeks of the experiment, stereological estimation of volume density and total volume of islets and beta cells, volume-weighted mean islet volume, mass of the islets and pancreas, and total number of islets were carried out. Administration of eucalyptus significantly decreased the weight loss and increase of water and food intake in the treated diabetic groups in comparison to the STZ-induced diabetic (D) group. Volume density and total volume of islets, volume-weighted mean islet volume, mass of islets, and mass of pancreas of both treated diabetic groups were higher than the D group. In TD2, these stereological parameters increased significantly compared to the D group (p < 0.001). Volume density and total volume of beta cells increased 21% and 65%, respectively, in the TD2 group, but it was not statistically significant compared to the diabetic group (p > 0.05). The results suggested that Eucalyptus globulus with a dose-dependent manner ameliorates diabetic states by partial restoration of pancreatic beta cells and repair of STZ-induced damage in rats. This study suggests a beneficial effect of eucalyptus in the treatment of diabetes.
蓝桉叶在传统医学中用于治疗糖尿病。本研究旨在通过体视学方法评估蓝桉对链脲佐菌素（STZ）诱导的胰岛损伤的影响。选取50只体重200 - 250 g的成年血糖正常雄性Wistar大鼠，随机分为5组（每组n = 10）：对照组；STZ诱导糖尿病组（D组）——腹腔注射60 mg/kg链脲佐菌素；处理对照组（TC组）；处理糖尿病组（TD1组、TD2组），糖尿病诱导一周后，分别在饮食中给予20 g/kg和62.5 g/kg的蓝桉，并在饮用水中给予2.5 g/L的蓝桉水提取物。实验四周后，对胰岛和β细胞的体积密度、总体积、体积加权平均胰岛体积、胰岛和胰腺质量以及胰岛总数进行体视学评估。与STZ诱导糖尿病（D）组相比，给予蓝桉显著降低了处理糖尿病组的体重减轻以及水和食物摄入量的增加。两个处理糖尿病组的胰岛体积密度、总体积、体积加权平均胰岛体积、胰岛质量和胰腺质量均高于D组。在TD2组中，这些体视学参数与D组相比显著增加（p < 0.001）。TD2组β细胞的体积密度和总体积分别增加了21%和65%，但与糖尿病组相比无统计学意义（p > 0.05）。结果表明，蓝桉通过部分恢复大鼠胰腺β细胞和修复STZ诱导的损伤，以剂量依赖方式改善糖尿病状态。本研究表明蓝桉在糖尿病治疗中具有有益作用。
