Souza Stephanie S R, Smith Joshua T, Marcovici Michael M, Eckhardt Elissa M, Hansel Nicole B, Martin Isabella W, Andam Cheryl P
Department of Biological Sciences, University at Albany, State University of New York, Albany, New York, NY USA.
Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH USA.
NPJ Antimicrob Resist. 2024;2(1):14. doi: 10.1038/s44259-024-00032-9. Epub 2024 May 7.
in the bloodstream causes high morbidity and mortality, exacerbated by the spread of multidrug-resistant and methicillin-resistant (MRSA). We aimed to characterize the circulating lineages of from bloodstream infections and the contribution of individual lineages to resistance over time. Here, we generated 852 high-quality short-read draft genome sequences of isolates from patient blood cultures in a single hospital from 2010 to 2022. A total of 80 previously recognized sequence types (ST) and five major clonal complexes are present in the population. Two frequently detected lineages, ST5 and ST8 exhibited fluctuating demographic structures throughout their histories. The rise and fall in their population growth coincided with the acquisition of antimicrobial resistance, mobile genetic elements, and superantigen genes, thus shaping the accessory genome structure across the entire population. These results reflect undetected selective events and changing ecology of multidrug-resistant in the bloodstream.
在血流中会导致高发病率和死亡率，耐多药和耐甲氧西林（MRSA）的传播使其情况更加恶化。我们旨在表征血流感染中分离株的循环谱系以及各个谱系随时间推移对耐药性的贡献。在此，我们生成了2010年至2022年一家医院患者血培养中分离株的852个高质量短读长草图基因组序列。该群体中共有80种先前已识别的序列类型（ST）和五个主要克隆复合体。两个经常检测到的谱系，ST5和ST8在其整个历史中呈现出波动的种群结构。它们种群增长的起伏与抗菌药物耐药性、移动遗传元件和超抗原基因的获得相吻合，从而塑造了整个人口中的辅助基因组结构。这些结果反映了血流中未被检测到的选择性事件以及耐多药分离株不断变化的生态。
