• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蛋白磷酸酶 2C 族 A 型 TaPP2CA 与钙依赖性蛋白激酶 TaCDPK5/TaCDPK9-1 相互作用，后者磷酸化来自小麦（Triticum aestivum L.）的 TabZIP60 转录因子。

The protein phosphatase 2C clade A TaPP2CA interact with calcium-dependent protein kinases, TaCDPK5/TaCDPK9-1, that phosphorylate TabZIP60 transcription factor from wheat (Triticum aestivum L.).

机构信息

College of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China.

College of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China.

出版信息

Plant Sci. 2022 Aug;321:111304. doi: 10.1016/j.plantsci.2022.111304. Epub 2022 May 6.

DOI:10.1016/j.plantsci.2022.111304
PMID:35696905
Abstract

Previously we have found that TabZIP60 from the ABF/AREB (ABRE-binding factor/ABA-responsive element-binding protein) subfamily of bZIP transcription factor (TF) was involved in salt stress response. However, the regulatory mechanism of TabZIP60 is unknown. In the present study, we identified two calcium-dependent protein kinase (CDPK) genes, TaCDPK5/TaCDPK9-1, which were clustered into group Ⅰ and were induced by salt, abscisic acid (ABA), and polyethylene glycol (PEG) treatments. RT-qPCR results showed that the expression level of salt-induced TabZIP60 was drastically inhibited by Ca channel blocker LaCl. TaCDPK5/TaCDPK9-1 were involved in interaction with TabZIP60 protein in vivo and in vitro. And TaCDPK5/TaCDPK9-1 could autophosphorylate and phosphorylate TabZIP60 protein in a Ca-dependent way. Mutational analysis indicated that Serine-110 of TabZIP60 was essential for TaCDPK5/TaCDPK9-1-TabZIP60 interaction and was the phosphorylation site of TaCDPK5/TaCDPK9-1 kinases. Yeast two-hybrid assay results showed the interactions between TaCDPK5/TaCDPK9-1 and wheat protein phosphatase 2 C clade A TaPP2CA116/ TaPP2CA121 separately. These findings demonstrate that the phosphorylation status of TabZIP60 controlled by TaPP2CA116/ TaPP2CA121 and TaCDPK5/TaCDPK9-1 might play a crucial role in wheat during salt stress.

摘要

先前，我们发现 ABF/AREB（ABRE 结合因子/ABA 响应元件结合蛋白）bZIP 转录因子（TF）亚家族中的 TabZIP60 参与了盐胁迫反应。然而，TabZIP60 的调控机制尚不清楚。在本研究中，我们鉴定了两个钙依赖性蛋白激酶（CDPK）基因，TaCDPK5/TaCDPK9-1，它们被聚类到第Ⅰ组，并且受盐、脱落酸（ABA）和聚乙二醇（PEG）处理诱导。RT-qPCR 结果表明，盐诱导的 TabZIP60 的表达水平被 Ca 通道阻滞剂 LaCl 显著抑制。TaCDPK5/TaCDPK9-1 参与了 TabZIP60 蛋白在体内和体外的相互作用。并且 TaCDPK5/TaCDPK9-1 可以以 Ca 依赖性方式自我磷酸化和磷酸化 TabZIP60 蛋白。突变分析表明，TabZIP60 的丝氨酸-110 是 TaCDPK5/TaCDPK9-1-TabZIP60 相互作用所必需的，并且是 TaCDPK5/TaCDPK9-1 激酶的磷酸化位点。酵母双杂交试验结果表明 TaCDPK5/TaCDPK9-1 分别与小麦蛋白磷酸酶 2C 家族 A TaPP2CA116/TaPP2CA121 互作。这些发现表明，由 TaPP2CA116/TaPP2CA121 和 TaCDPK5/TaCDPK9-1 控制的 TabZIP60 的磷酸化状态可能在小麦的盐胁迫过程中发挥关键作用。

相似文献

1
The protein phosphatase 2C clade A TaPP2CA interact with calcium-dependent protein kinases, TaCDPK5/TaCDPK9-1, that phosphorylate TabZIP60 transcription factor from wheat (Triticum aestivum L.).蛋白磷酸酶 2C 族 A 型 TaPP2CA 与钙依赖性蛋白激酶 TaCDPK5/TaCDPK9-1 相互作用，后者磷酸化来自小麦（Triticum aestivum L.）的 TabZIP60 转录因子。
Plant Sci. 2022 Aug;321:111304. doi: 10.1016/j.plantsci.2022.111304. Epub 2022 May 6.
2
TabZIP60 is involved in the regulation of ABA synthesis-mediated salt tolerance through interacting with TaCDPK30 in wheat (Triticum aestivum L.).TabZIP60 通过与 TaCDPK30 互作参与调控 ABA 合成介导的小麦耐盐性。
Planta. 2023 May 2;257(6):107. doi: 10.1007/s00425-023-04141-z.
3
Wheat TabZIP8, 9, 13 participate in ABA biosynthesis in NaCl-stressed roots regulated by TaCDPK9-1.小麦 TabZIP8、9、13 通过 TaCDPK9-1 参与 NaCl 胁迫下根系 ABA 生物合成调控。
Plant Physiol Biochem. 2020 Jun;151:650-658. doi: 10.1016/j.plaphy.2020.03.039. Epub 2020 Apr 17.
4
A novel wheat bZIP transcription factor, TabZIP60, confers multiple abiotic stress tolerances in transgenic Arabidopsis.一种新型的小麦 bZIP 转录因子 TabZIP60，可在转基因拟南芥中赋予多种非生物胁迫耐受性。
Physiol Plant. 2015 Apr;153(4):538-54. doi: 10.1111/ppl.12261. Epub 2014 Oct 18.
5
Reducing expression of a nitrate-responsive bZIP transcription factor increases grain yield and N use in wheat.降低硝酸盐响应型 bZIP 转录因子的表达可提高小麦的产量和氮素利用。
Plant Biotechnol J. 2019 Sep;17(9):1823-1833. doi: 10.1111/pbi.13103. Epub 2019 Mar 21.
6
Direct interactions of ABA-insensitive(ABI)-clade protein phosphatase(PP)2Cs with calcium-dependent protein kinases and ABA response element-binding bZIPs may contribute to turning off ABA response.ABA 不敏感（ABI）蛋白磷酸酶（PP）2C 与钙依赖性蛋白激酶和 ABA 反应元件结合 bZIP 的直接相互作用可能有助于关闭 ABA 反应。
Plant Mol Biol. 2012 Dec;80(6):647-58. doi: 10.1007/s11103-012-9973-3. Epub 2012 Sep 25.
7
Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis.在拟南芥中，有三种 SnRK2 蛋白激酶是响应水分胁迫的脱落酸信号的主要正向调节因子。
Plant Cell Physiol. 2009 Dec;50(12):2123-32. doi: 10.1093/pcp/pcp147.
8
Pivotal role of the AREB/ABF-SnRK2 pathway in ABRE-mediated transcription in response to osmotic stress in plants.该 AREB/ABF-SnRK2 途径在植物响应渗透胁迫的 ABRE 介导转录中起关键作用。
Physiol Plant. 2013 Jan;147(1):15-27. doi: 10.1111/j.1399-3054.2012.01635.x. Epub 2012 May 16.
9
Identification of upstream transcription factors and an interacting PP2C protein of dehydrin gene in wheat.鉴定小麦脱水素基因的上游转录因子和互作的 PP2C 蛋白。
Plant Signal Behav. 2019;14(12):1678370. doi: 10.1080/15592324.2019.1678370. Epub 2019 Oct 12.
10
ABRE-BINDING FACTORS play a role in the feedback regulation of ABA signaling by mediating rapid ABA induction of ABA co-receptor genes.ABRE-BINDING FACTORS 在 ABA 信号的反馈调节中发挥作用，通过介导 ABA 对 ABA 共受体基因的快速诱导。
New Phytol. 2019 Jan;221(1):341-355. doi: 10.1111/nph.15345. Epub 2018 Jul 18.

引用本文的文献

1
Walnut phosphatase 2A proteins interact with basic leucine zipper protein JrVIP1 to regulate osmotic stress response calcium signaling.核桃磷酸酶2A蛋白与碱性亮氨酸拉链蛋白JrVIP1相互作用，以调节渗透胁迫反应中的钙信号传导。
For Res (Fayettev). 2024 May 6;4:e016. doi: 10.48130/forres-0024-0012. eCollection 2024.
2
Transcriptional Landscape of Cotton Roots in Response to Salt Stress at Single-cell Resolution.单细胞分辨率下棉花根系对盐胁迫响应的转录图谱
Plant Commun. 2023 Oct 27;5(2):100740. doi: 10.1016/j.xplc.2023.100740.
3
Insights into Salinity Tolerance in Wheat.
小麦耐盐性研究进展。
Genes (Basel). 2024 Apr 29;15(5):573. doi: 10.3390/genes15050573.
4
Characterization of putative calcium-dependent protein kinase-1 () gene: hubs in signalling and tolerance network of wheat under terminal heat.假定的钙依赖性蛋白激酶-1（）基因的特征：小麦在高温胁迫下信号传导和耐受性网络中的枢纽
3 Biotech. 2024 Jun;14(6):150. doi: 10.1007/s13205-024-03989-6. Epub 2024 May 7.
5
Calcium signaling-mediated transcriptional reprogramming during abiotic stress response in plants.钙信号介导的植物非生物胁迫响应中的转录重编程。
Theor Appl Genet. 2023 Sep 20;136(10):210. doi: 10.1007/s00122-023-04455-2.
6
TabZIP60 is involved in the regulation of ABA synthesis-mediated salt tolerance through interacting with TaCDPK30 in wheat (Triticum aestivum L.).TabZIP60 通过与 TaCDPK30 互作参与调控 ABA 合成介导的小麦耐盐性。
Planta. 2023 May 2;257(6):107. doi: 10.1007/s00425-023-04141-z.
7
Silencing of the calcium-dependent protein kinase TaCDPK27 improves wheat resistance to powdery mildew.钙依赖蛋白激酶 TaCDPK27 的沉默提高了小麦对白粉病的抗性。
BMC Plant Biol. 2023 Mar 8;23(1):134. doi: 10.1186/s12870-023-04140-y.
8
Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry ().全基因组鉴定、特征分析和木本草莓（）Tubby-like 蛋白（TLP）基因家族成员的表达分析
Int J Mol Sci. 2022 Oct 8;23(19):11961. doi: 10.3390/ijms231911961.