Koike Seiichi, Keino-Masu Kazuko, Masu Masayuki
Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Department of Molecular Neurobiology, Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
MicroPubl Biol. 2024 Apr 24;2024. doi: 10.17912/micropub.biology.001212. eCollection 2024.
Autotaxin, encoded by the gene, produces lysophosphatidic acid (LPA), which exerts numerous biological functions via its cognate receptors. null mutant mice die by embryonic day 9.5 owing to aberrant vascular development in the yolk sac, preventing analysis after that period. In this study, we found that heterozygous mice in the DBA/2 genetic background showed an eye-open-at-birth phenotype at high frequency, caused by failure of eyelid closure during the embryonic stage. Notably, wildtype pups from the heterozygous dam showed the phenotype, although at lower frequency, suggesting that maternal LPA affects the embryonic development.
由该基因编码的自分泌运动因子产生溶血磷脂酸（LPA），LPA通过其同源受体发挥多种生物学功能。基因敲除突变小鼠在胚胎第9.5天死亡，原因是卵黄囊血管发育异常，在此之后无法进行分析。在本研究中，我们发现DBA/2遗传背景下的杂合小鼠高频出现出生时睁眼的表型，这是由胚胎期眼睑闭合失败所致。值得注意的是，来自杂合母鼠的野生型幼崽也表现出该表型，尽管频率较低，这表明母体LPA会影响胚胎发育。
