一种用于检测D-丙氨酸羧肽酶以及β-内酰胺酶酯酶活性的直接分光光度法。
A direct spectrophotometric assay for D-alanine carboxypeptidases and for the esterase activity of beta-lactamases.
作者信息
Pratt R F, Faraci W S, Govardhan C P
出版信息
Anal Biochem. 1985 Jan;144(1):204-6. doi: 10.1016/0003-2697(85)90106-x.
PMID:3872609
Abstract
A direct spectrophotometric pH indicator method has been devised to assay the activity of the D-Ala carboxypeptidase/transpeptidase of Streptomyces R61, and which should be of general application to D-Ala carboxypeptidases. The substrate employed is N,N'-diacetyl-L-lysyl-D-alanyl-D-lactate. The method allows the determination of steady-state kinetic parameters, and can also be used for the assay of the esterase activity of beta-lactamases against specific depsipeptides.
摘要
已设计出一种直接分光光度法pH指示剂法来测定链霉菌R61的D-丙氨酸羧肽酶/转肽酶的活性，该方法应可普遍应用于D-丙氨酸羧肽酶。所使用的底物是N，N'-二乙酰-L-赖氨酰-D-丙氨酰-D-乳酸酯。该方法可用于测定稳态动力学参数，也可用于测定β-内酰胺酶对特定缩肽的酯酶活性。
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