Laboratorio de Nematología, Escuela de Ciencias Agrarias, Universidad Nacional, 86-3000Heredia, Costa Rica.
Institute for Sustainable Agriculture, Department of Crop Protection, Avenida Menéndez Pidal s/n, 14004 Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, Spain.
J Helminthol. 2024 May 10;98:e39. doi: 10.1017/S0022149X24000294.
During nematode surveys of natural vegetation in forests of La Cima de Copey de Dota, San José, San José province, Costa Rica, a species closely resembling and related species was found. Integrative taxonomical approaches demonstrated that it is a new species described herein as sp. nov. The new species is parthenogenetic (only females have been detected) and characterised by a short body (276-404 μm); lip region with two annuli, not offset, not separated from body contour; first lip annulus partially covering the second lip annulus. Stylet thin, very long (113-133 μm) and flexible, occupying 30.5-47.8% of body length. Excretory pore located from one or two annuli anterior to one or two annuli posterior to level of stylet knobs, at 42 (37-45) μm from anterior end. Female genital tract monodelphic, prodelphic, outstretched, and occupying 35-45% of body length, with vagina slightly ventrally curved (14-18 μm long). Anus located 6-11 annuli from the tail terminus. Tail conoid and bluntly rounded terminus, the last 2-3 annuli oriented dorsally. Results of molecular characterisation and phylogenetic analyses of D2-D3 expansion segments of 28S rRNA, ITS, and partial 18S rRNA, as well as cytochrome oxidase c subunit 1 gene sequences further characterised the new species and clearly separated it from and other related species (, , and ).
在哥斯达黎加圣何塞省拉西马德科佩的科佩的自然植被中的线虫调查中，发现了一种与 非常相似的相关物种。综合分类学方法表明，这是一种新物种，本文将其描述为 sp. nov.。该新种是孤雌生殖的（仅发现雌性），其特征为身体短（276-404 μm）；唇区有两个环，没有偏移，与身体轮廓没有分开；第一唇环部分覆盖第二唇环。口针纤细，非常长（113-133 μm）且有弹性，占身体长度的 30.5-47.8%。排泄孔位于口针棘前 1-2 个环或后 1-2 个环，距离前端 42（37-45）μm。雌性生殖道单胚，原胚，伸展，占身体长度的 35-45%，阴道略向腹侧弯曲（长 14-18 μm）。肛门位于尾部末端 6-11 个环处。尾部圆锥形，末端钝圆，最后 2-3 个环向背侧。28S rRNA、ITS 和部分 18S rRNA 的 D2-D3 扩展片段以及细胞色素氧化酶 c 亚基 1 基因序列的分子特征和系统发育分析进一步描述了该新种，并将其与 和其他相关物种（ 、 和 ）清楚地区分开来。
