亚甲基四氢叶酸脱氢酶-亚甲基四氢叶酸环化水解酶-甲酰四氢叶酸合成酶。脱氢酶-环化水解酶活性位点的亲和标记 。
Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate-cyclohydrolase-formyltetrahy dro folate synthetase. Affinity labelling of the dehydrogenase-cyclohydrolase active site.
作者信息
Smith D D, MacKenzie R E
出版信息
Biochem Biophys Res Commun. 1985 Apr 16;128(1):148-54. doi: 10.1016/0006-291x(85)91657-2.
PMID:3872659
Abstract
Methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase are inactivated in parallel by carbodiimide-activated folic acid in an NADP-dependent reaction. Modification with tritium-labelled reagent resulted in the incorporation of 1 mole 3H-folate per mole polypeptide, which demonstrates that these activities share a single folate binding site.
摘要
亚甲基四氢叶酸脱氢酶和亚胺甲基四氢叶酸环化水解酶在依赖于烟酰胺腺嘌呤二核苷酸磷酸（NADP）的反应中，被碳二亚胺活化的叶酸同时失活。用氚标记的试剂进行修饰，导致每摩尔多肽掺入1摩尔3H-叶酸，这表明这些活性共享一个单一的叶酸结合位点。
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