Han Xue, Shi Fan, Guo Shujun, Li Yao, Wang Hongtao, Song Chuanwang, Wu Shiwu
Department of Immunology, School of Laboratory Medicine, Bengbu Medical University, Bengbu, 233030, China.
Anhui Provincial Key Laboratory of Infection and Immunity, Bengbu Medical University, Bengbu, 233030, China.
Curr Pharm Biotechnol. 2025;26(1):120-131. doi: 10.2174/0113892010290582240419051056.
Breast Cancer (BC) is a female malignancy with a high mortality rate. Novel diagnostic and prognostic biomarkers are valuable for reducing BC mortality. Our study is designed to undrape the precise role of the LINC00466/miR-4731-5p/EPHA2 axis in BC.
The Cancer Genome Atlas (TCGA) sequencing dataset was utilized to compare the levels of LINC00466. The levels of LINC00466, miR-4731-5p, and EPHA2 were tested by qRTPCR. Cell proliferation and cycle were detected by CCK-8 assay and flow cytometer. role of LINC00466 was tested by Xenograft nude models. Binding sites were predicted by TargetScan and Starbase. The binding relationship was employed by Dual-luciferase reporter gene assay and RNA pull-down assay.
LINC00466 was increased in human breast cancer tissues. LINC00466 was negatively associated with miR-4731-5p and positively correlated with EPHA2 in human breast cancer tissues. Down-regulation of LINC00466 suppressed the proliferation and arrested the cell cycle of breast cancer cells, and inhibited tumor growth .
LINC00466 promoted BC development via mediating the miR-4731-5p/EPHA2 axis, which has the potential value as a promising therapeutic target in BC.
乳腺癌（BC）是一种死亡率很高的女性恶性肿瘤。新型诊断和预后生物标志物对于降低乳腺癌死亡率具有重要价值。我们的研究旨在揭示LINC00466/miR-4731-5p/EPHA2轴在乳腺癌中的精确作用。
利用癌症基因组图谱（TCGA）测序数据集比较LINC00466的水平。通过qRTPCR检测LINC00466、miR-4731-5p和EPHA2的水平。采用CCK-8法和流式细胞仪检测细胞增殖和细胞周期。通过异种移植裸鼠模型检测LINC00466的作用。通过TargetScan和Starbase预测结合位点。采用双荧光素酶报告基因检测和RNA下拉实验确定结合关系。
LINC00466在人乳腺癌组织中表达升高。在人乳腺癌组织中，LINC00466与miR-4731-5p呈负相关，与EPHA2呈正相关。下调LINC00466可抑制乳腺癌细胞的增殖，使细胞周期停滞，并抑制肿瘤生长。
LINC00466通过介导miR-4731-5p/EPHA2轴促进乳腺癌发展，其具有作为乳腺癌有前景治疗靶点的潜在价值。
