长链非编码RNA LINC00466通过miR-4731-5p/EPHA2途径促进乳腺癌进展。

LncRNA LINC00466 Promotes the Progression of Breast Cancer miR-4731-5p/EPHA2 Pathway.

作者信息

Han Xue, Shi Fan, Guo Shujun, Li Yao, Wang Hongtao, Song Chuanwang, Wu Shiwu

机构信息

Department of Immunology, School of Laboratory Medicine, Bengbu Medical University, Bengbu, 233030, China.

Anhui Provincial Key Laboratory of Infection and Immunity, Bengbu Medical University, Bengbu, 233030, China.

出版信息

Curr Pharm Biotechnol. 2025;26(1):120-131. doi: 10.2174/0113892010290582240419051056.

DOI:10.2174/0113892010290582240419051056

PMID:38726776

Abstract

BACKGROUND

Breast Cancer (BC) is a female malignancy with a high mortality rate. Novel diagnostic and prognostic biomarkers are valuable for reducing BC mortality. Our study is designed to undrape the precise role of the LINC00466/miR-4731-5p/EPHA2 axis in BC.

METHODS

The Cancer Genome Atlas (TCGA) sequencing dataset was utilized to compare the levels of LINC00466. The levels of LINC00466, miR-4731-5p, and EPHA2 were tested by qRTPCR. Cell proliferation and cycle were detected by CCK-8 assay and flow cytometer. role of LINC00466 was tested by Xenograft nude models. Binding sites were predicted by TargetScan and Starbase. The binding relationship was employed by Dual-luciferase reporter gene assay and RNA pull-down assay.

RESULTS

LINC00466 was increased in human breast cancer tissues. LINC00466 was negatively associated with miR-4731-5p and positively correlated with EPHA2 in human breast cancer tissues. Down-regulation of LINC00466 suppressed the proliferation and arrested the cell cycle of breast cancer cells, and inhibited tumor growth .

CONCLUSION

LINC00466 promoted BC development via mediating the miR-4731-5p/EPHA2 axis, which has the potential value as a promising therapeutic target in BC.

摘要

背景

乳腺癌（BC）是一种死亡率很高的女性恶性肿瘤。新型诊断和预后生物标志物对于降低乳腺癌死亡率具有重要价值。我们的研究旨在揭示LINC00466/miR-4731-5p/EPHA2轴在乳腺癌中的精确作用。

方法

利用癌症基因组图谱（TCGA）测序数据集比较LINC00466的水平。通过qRTPCR检测LINC00466、miR-4731-5p和EPHA2的水平。采用CCK-8法和流式细胞仪检测细胞增殖和细胞周期。通过异种移植裸鼠模型检测LINC00466的作用。通过TargetScan和Starbase预测结合位点。采用双荧光素酶报告基因检测和RNA下拉实验确定结合关系。