Godeau F, Ishizaka T, Koide S S
Cell Differ. 1985 Feb;16(1):35-41. doi: 10.1016/0045-6039(85)90605-0.
Progesterone induced a transient increase in the incorporation of [3H]methyl groups into phospholipids of Xenopus oocytes followed by a rise in 45Ca2+ uptake. Phospholipid methylation reached a maximum as early as 15 s after progesterone treatment and returned to basal level within 2 min. Steroids inactive in promoting oocyte maturation were less effective in affecting phospholipid methylation. Methyltransferase inhibitors, 3-deaza-SIBA, SIBA, and Sinefungin, inhibited progesterone-activated stimulation of phospholipid methylation, calcium uptake and meiotic maturation. Phospholipid methylation is the earliest detectable biochemical event occurring in oocytes after exposure to progesterone followed by calcium influx and leading to germinal vesicle dissolution.
孕酮诱导非洲爪蟾卵母细胞磷脂中[3H]甲基掺入量短暂增加，随后45Ca2+摄取量上升。磷脂甲基化在孕酮处理后最早15秒达到最大值，并在2分钟内恢复到基础水平。在促进卵母细胞成熟方面无活性的类固醇对磷脂甲基化的影响较小。甲基转移酶抑制剂3-脱氮-SIBA、SIBA和杀稻瘟菌素抑制孕酮激活的磷脂甲基化、钙摄取和减数分裂成熟刺激。磷脂甲基化是卵母细胞暴露于孕酮后最早可检测到的生化事件，随后是钙内流并导致生发泡溶解。
