College of Forestry, Shandong Agricultural University, Tai'an 271018, China.
College of Forestry, Shandong Agricultural University, Tai'an 271018, China.
Ecotoxicol Environ Saf. 2024 Jun 15;278:116434. doi: 10.1016/j.ecoenv.2024.116434. Epub 2024 May 9.
The growing use of nanomaterials has sparked significant interest in assessing the insect toxicities of nanoparticles. The silkworm, as an economically important insect, serves as a promising model for studying how insects respond to harmful substances. Here, we conducted a comprehensive investigation on the impact of graphene oxide (GO) on silkworms using a combination of physiological and transcriptome analyses. GO can enter the midguts and posterior silk glands of silkworms. High GO concentrations (> 25 mg/L) significantly (P < 0.01) inhibited larval growth. Additionally, GO (> 5 mg/L) significantly reduced the cocooning rate, and GO (> 15 mg/L) hindered oviduct development and egg laying in silkworms. GO increased the reactive oxygen species content and regulated catalase activity, suggesting that it may affect insect growth by regulating reactive oxygen detoxification. The transcriptome data analysis showed that 35 metabolism-related genes and 20 ribosome biogenesis-related genes were differentially expressed in response to GO, and their expression levels were highly correlated. Finally, we propose that a Ribosome biogenesis-Metabolic signaling network is involved in responses to GO. The research provides a new perspective on the molecular responses of insects to GO.
纳米材料的应用日益广泛，促使人们对评估纳米颗粒对昆虫的毒性产生了浓厚的兴趣。家蚕作为一种具有重要经济价值的昆虫，是研究昆虫如何应对有害物质的理想模型。在这里，我们采用生理和转录组分析相结合的方法，全面研究了氧化石墨烯（GO）对家蚕的影响。GO 可以进入家蚕的中肠和后部丝腺。高浓度的 GO（>25mg/L）显著（P<0.01）抑制了幼虫的生长。此外，GO（>5mg/L）显著降低了化蛹率，GO（>15mg/L）阻碍了家蚕输卵管的发育和产卵。GO 增加了活性氧物质的含量并调节了过氧化氢酶的活性，表明它可能通过调节活性氧解毒来影响昆虫的生长。转录组数据分析表明，35 个与代谢相关的基因和 20 个与核糖体生物发生相关的基因对 GO 有差异表达，并且它们的表达水平高度相关。最后，我们提出了一个核糖体生物发生-代谢信号网络参与了对 GO 的响应。该研究为昆虫对 GO 的分子响应提供了新的视角。
