Department of Biochemistry, College of Medicine, University of Ha'il, Hail 81411, Saudi Arabia; Medical and Diagnostic Research Centre, University of Ha'il, Hail 55473, Saudi Arabia.
Department of Biosciences, Jamia Millia Islamia, Okhla, New Delhi 110025, India.
Biochim Biophys Acta Mol Cell Biol Lipids. 2024 Aug;1869(6):159505. doi: 10.1016/j.bbalip.2024.159505. Epub 2024 May 8.
Tylophora indica (Burm f.) Merrill, belong to family Asclepiadaceae, is considered to be a natural remedy with high medicinal benefits. The objective of this work is to assess the metabolomic profile of T. indica leaves enriched in alkaloids, as well as to evaluate the in vitro cytotoxicity of these leaves using the MTT assay on human breast MCF-7 and liver HepG2 cancer cell lines. Dried leaves of T. indica were extracted by sonication, using methanol containing 2 % (v/v) of acetic acid and obtained fraction was characterized by HPTLC and UPLC-MS. The UPLC-MS study yielded a preliminary identification of 32 metabolites, with tylophorine, tylophorine B, tylophorinine, and tylophorinidine being the predominant metabolites. The cytotoxicity of the extract of T. indica was evaluated on HepG2 and MCF-7 cell lines, yielding inhibitory concentration (IC) values of 75.71 μg/mL and 69.60 μg/mL, respectively. Data suggested that the phytochemical screening clearly showed presence of numerous secondary metabolites with moderate cytotoxic efficacy. In conclusion, the future prospects of T. indica appear promising for the advancement of phytopharmaceutical-based anticancer medications, as well as for the design of contemporary pharmaceuticals in the field of cancer chemotherapy.
印度娃儿藤（Burm f.）Merrill，属于萝藦科，被认为是一种具有高药用价值的天然药物。本工作的目的是评估富含生物碱的印度娃儿藤叶的代谢组学特征，并使用 MTT 法在人乳腺癌 MCF-7 和肝癌 HepG2 癌细胞系上评估这些叶子的体外细胞毒性。通过超声处理，使用含 2%（v/v）乙酸的甲醇对印度娃儿藤的干燥叶子进行提取，并通过 HPTLC 和 UPLC-MS 对获得的部分进行表征。UPLC-MS 研究初步鉴定出 32 种代谢物，其中主要代谢物为娃儿藤碱、娃儿藤碱 B、娃儿藤宁和娃儿藤定碱。印度娃儿藤提取物对 HepG2 和 MCF-7 细胞系的细胞毒性进行了评估，得出的抑制浓度（IC）值分别为 75.71μg/mL 和 69.60μg/mL。数据表明，植物化学筛选清楚地表明存在许多具有中等细胞毒性的次生代谢物。总之，印度娃儿藤的未来前景似乎很有希望用于推进基于植物药的抗癌药物，以及用于癌症化疗领域的现代药物设计。
