das Graças Diego Assis, de Morais Eziquiel, Lima Alyne C S, de Souza Shirley M, Sousa Luciano F, Franco Diego C, Silva Artur L C, Silva André G Maciel E
Laboratório de Engenharia Biológica, Guamá Science and Technology Park, Belém 66075-110, PA, Brazil.
Instituto Federal de Ciência e Tecnologia do Pará, Castanhal 29056-264, PA, Brazil.
Animals (Basel). 2024 Apr 24;14(9):1269. doi: 10.3390/ani14091269.
This study aimed to assess the impact of palm oil deodorizer distillate (POD) on the ruminal environment, including (i) microbial community, (ii) ruminal degradability, and (iii) apparent digestibility in sheep. The data used were derived from twenty rumen-cannulated sheep fed five isoproteic and isofiber diets based on elephant grass ( Schum. cv. Roxo) silage supplemented with 0, 25, 50, 75, or 100 g kg POD on a dry matter (DM) basis. Rumen fluid samples were collected three hours after feeding directly from the ventral sac of the rumen via a cannula and then subjected to DNA extraction, which was subsequently used for 16S rDNA amplification, followed by sequencing and diversity analysis. In this study, the microbial diversity was dominated by Bacteroidetes and Firmicutes, followed by Euryarchaetoa, Actinobacteria, and Tenericutes, in the ruminal environment, and was slightly modified when supplemented with the POD up to 100 g/kg (10%), leading to only a slight decrease in the diversity index. The ruminal degradability, ruminal fermentation parameters, and apparent digestibility were slightly compromised by the inclusion of up to 25 g of POD per kg of DM, and larger inclusions interfered with the ruminal degradability of fibrous fractions and the apparent digestibility of dry matter. This lipid supplement showed good results for feeding sheep and is an inexpensive and abundant alternative in the regional market.
本研究旨在评估棕榈油脱臭馏出物（POD）对绵羊瘤胃环境的影响，包括（i）微生物群落、（ii）瘤胃降解率以及（iii）表观消化率。所使用的数据来自20只安装了瘤胃瘘管的绵羊，这些绵羊饲喂了五种等蛋白、等纤维的日粮，日粮以象草（Schum. cv. Roxo）青贮料为基础，按干物质（DM）计分别添加了0、25、50、75或100 g/kg的POD。在喂食后3小时，通过瘘管直接从瘤胃腹囊采集瘤胃液样本，然后进行DNA提取，随后用于16S rDNA扩增，接着进行测序和多样性分析。在本研究中，瘤胃环境中的微生物多样性以拟杆菌门和厚壁菌门为主，其次是广古菌门、放线菌门和柔膜菌门，当添加高达100 g/kg（10%）的POD时，微生物多样性略有改变，导致多样性指数仅略有下降。每千克DM中添加高达25 g的POD会使瘤胃降解率、瘤胃发酵参数和表观消化率略有降低，而更大剂量的添加会干扰纤维组分的瘤胃降解率和干物质的表观消化率。这种脂质补充剂在饲喂绵羊方面显示出良好的效果，并且在区域市场上是一种廉价且丰富的替代品。
