Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSPC), Universidad de La Laguna, 38029 San Cristóbal de La Laguna, Spain.
HLA Laboratory, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, Mexico.
Int J Mol Sci. 2024 Apr 24;25(9):4632. doi: 10.3390/ijms25094632.
COVID-19 is characterized by a wide range of clinical manifestations, where aging, underlying diseases, and genetic background are related to worse outcomes. In the present study, the differential expression of seven genes related to immunity, , , , , , , and , was analyzed in individuals with COVID-19 diagnoses of different disease severities. Two-step RT-qPCR was performed to determine the relative gene expression in whole-blood samples from 160 individuals. The expression of ( < 0.05) and ( < 0.05) was higher in moderate hospitalized cases than in severe ones. Increased gene expression of (OR = 0.64, CI = 0.52-0.79; = 0.001), (OR = 0.581, CI = 0.43-0.79; = 0.001), and (OR = 0.544, CI = 0.39-0.69; < 0.001) was associated with a lower risk of requiring IMV. Moreover, (OR = 0.646, CI = 0.50-0.83; = 0.001), (OR = 0.57, CI = 0.39-0.83; = 0.003), (OR = 0.80, CI = 0.653-0.979; = 0.03), and (OR = 0.827, CI = 0.69-0.991; = 0.039) expression was associated with patient survival. In conclusion, the relevance of , , and in controlling the viral infection was confirmed.
新型冠状病毒肺炎（COVID-19）的临床表现多种多样，衰老、基础疾病和遗传背景与较差的预后相关。在本研究中，分析了与免疫相关的七个基因在不同疾病严重程度 COVID-19 诊断个体中的差异表达。采用两步 RT-qPCR 法测定了 160 例个体全血样本中的相对基因表达。中度住院病例的 （<0.05）和 （<0.05）表达高于重度住院病例。基因表达增加的 （OR=0.64，CI=0.52-0.79；=0.001）、 （OR=0.581，CI=0.43-0.79；=0.001）和 （OR=0.544，CI=0.39-0.69；<0.001）与需要机械通气的风险降低相关。此外， （OR=0.646，CI=0.50-0.83；=0.001）、 （OR=0.57，CI=0.39-0.83；=0.003）、 （OR=0.80，CI=0.653-0.979；=0.03）和 （OR=0.827，CI=0.69-0.991；=0.039）的表达与患者生存相关。总之，证实了 、 、 和 在控制病毒感染中的相关性。
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