Domínguez-de-Barros Angélica, Sirvent-Blanco Candela, García-Pérez Omar, Gajate-Arenas Malena, García-Ramos Alma, Migliazzo Claudia, Piñero José E, Lorenzo-Morales Jacob, Córdoba-Lanús Elizabeth
Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSPC), Universidad de La Laguna, 38029 La Laguna, Tenerife, Spain.
Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain.
Int J Mol Sci. 2025 May 21;26(10):4934. doi: 10.3390/ijms26104934.
Oxidative stress and inflammation influence immune response and epigenetic mechanisms in infectious diseases. In mild COVID-19, host-encoded miRNA profiles remain underexplored, although they reveal mechanistic insights into disease pathogenesis. This study evaluated ageing and oxidative stress biomarkers (telomere length (TL), TBARS, 8-OHdG, and circulating related-miRNA expression) in 75 mild cases and 30 non-COVID-19 controls. TL correlated with age (R = -0.384, = 0.005) and was shorter in cases compared to controls (rTL 1.46 ± 0.51 vs. 0.99 ± 0.37; < 0.001), being similar between saliva and blood samples ( = 0.917). miR-138-5p was upregulated in COVID-19 cases ( = 0.026) and correlated with 8-OHdG (R = 0.403, = 0.05), which was increased in cases ( = 0.040); miR-210-3p was downregulated in infected individuals ( = 0.008), while miR-182-5p expression correlated with TBARS (R = 0.582, = 0.018). miR-34a-5p and miR155-5p expression was not altered in mild COVID-19. These findings suggest early systemic cellular damage in mild COVID-19 and highlight miR-138-5p and miR-182-5p as potential early biomarkers of oxidative stress.
氧化应激和炎症影响传染病中的免疫反应和表观遗传机制。在轻度新冠肺炎中，宿主编码的微小RNA（miRNA）谱仍未得到充分研究，尽管它们揭示了疾病发病机制的相关机制见解。本研究评估了75例轻度病例和30例非新冠肺炎对照者的衰老和氧化应激生物标志物（端粒长度（TL）、丙二醛（TBARS）、8-羟基脱氧鸟苷（8-OHdG）和循环相关miRNA表达）。TL与年龄相关（R = -0.384，P = 0.005），病例组的TL比对照组短（相对端粒长度[rTL]：1.46±0.51 vs. 0.99±0.37；P < 0.001），唾液和血液样本中的TL相似（P = 0.917）。miR-138-5p在新冠肺炎病例中上调（P = 0.026），并与8-OHdG相关（R = 0.403，P = 0.05），8-OHdG在病例组中升高（P = 0.040）；miR-210-3p在感染个体中下调（P = 0.008），而miR-182-5p表达与TBARS相关（R = 0.582，P = 0.018）。miR-34a-5p和miR-155-5p的表达在轻度新冠肺炎中未改变。这些发现表明轻度新冠肺炎存在早期全身性细胞损伤，并突出了miR-138-5p和miR-182-5p作为氧化应激潜在早期生物标志物的作用。
