Department of Urology, Ehime University Graduate School of Medicine, Toon 791-0295, Japan.
Human Biology Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
Int J Mol Sci. 2024 Apr 28;25(9):4818. doi: 10.3390/ijms25094818.
Intraductal carcinoma of the prostate (IDCP) has recently attracted increasing interest owing to its unfavorable prognoses. To effectively identify the IDCP-specific gene expression profile, we took a novel approach of characterizing a typical IDCP case using spatial gene expression analysis. A formalin-fixed, paraffin-embedded sample was subjected to Visium CytAssist Spatial Gene Expression analysis. IDCP within invasive prostate cancer sites was recognized as a distinct cluster separate from other invasive cancer clusters. Highly expressed genes defining the IDCP cluster, such as , , , and , reflected the aggressive nature of high-grade prostate cancer. IDCP sites also showed increased hypoxia markers , , , and ; decreased fibroblast markers , , and ; and decreased immune cell markers and . Overall, these findings indicate that the hypoxic tumor microenvironment and reduced recruitment of fibroblasts and immune cells, which reflect morphological features of IDCP, may influence the aggressiveness of high-grade prostate cancer.
前列腺管内癌（IDCP）由于其不良预后，最近引起了越来越多的关注。为了有效地识别 IDCP 的特定基因表达谱，我们采用了一种新的方法，通过空间基因表达分析来描述一个典型的 IDCP 病例。一个福尔马林固定、石蜡包埋的样本被用于 Visium CytAssist 空间基因表达分析。在侵袭性前列腺癌部位内的 IDCP 被识别为一个与其他侵袭性癌症簇分离的独特簇。定义 IDCP 簇的高表达基因，如 、 、 、 和 ，反映了高级别前列腺癌的侵袭性。IDCP 部位还表现出增加的缺氧标志物 、 、 和 ；减少的成纤维细胞标志物 、 、 和 ；以及减少的免疫细胞标志物 、 。总的来说，这些发现表明，低氧肿瘤微环境和减少的成纤维细胞和免疫细胞募集，反映了 IDCP 的形态特征，可能影响高级别前列腺癌的侵袭性。
