对5'-m7GMP和m7G(5')ppp(5')N具有特异性的人类自身抗体的检测

Detection of human autoantibodies specific for 5'-m7GMP and m7G(5')ppp(5')N.

作者信息

Munns T W, Liszewski M K, Freeman S K, Kaine J L

出版信息

Biochem Biophys Res Commun. 1985 Apr 30;128(2):1014-9. doi: 10.1016/0006-291x(85)90148-2.

Abstract

An enzyme-linked immunosorbent assay was utilized for the detection of spontaneously occurring antibodies with apparent specificities for m7G, 5'-m7GMP, and m7G(5')ppp(5')C. From the sera of 50 patients containing anti-nuclear antibodies, 48 (96%) possessed antibodies which bound to one or more immobilized nucleoside-BSA antigens (A-, G-, C-, U-, and T-BSA). Additionally, 8 (16%) of these sera contained immunoglobulins that reacted with m7G-BSA antigen. In these latter sera, soluble competitors such as m7G, 5'm7GMP, and m7G(5')ppp(5')C (but not 5'-AMP, -GMP, -CMP, -UMP, and -TMP or m1G and m22G) effectively inhibited antibody-binding to immobilized m7G-BSA. These results indicate the existence of spontaneously occurring anti-m7G antibodies in autoimmune diseases which are distinct from anti-G antibody populations.

摘要

采用酶联免疫吸附测定法检测对m7G、5'-m7GMP和m7G(5')ppp(5')C具有明显特异性的自发产生的抗体。在50例含有抗核抗体的患者血清中，48例（96%）拥有与一种或多种固定化核苷 - 牛血清白蛋白抗原（A - 、G - 、C - 、U - 和T - BSA）结合的抗体。此外，这些血清中有8例（16%）含有与m7G - BSA抗原反应的免疫球蛋白。在这些后一组血清中，可溶性竞争剂如m7G、5'm7GMP和m7G(5')ppp(5')C（但不是5'-AMP、-GMP、-CMP、-UMP和-TMP或m1G和m22G）有效地抑制了抗体与固定化m7G - BSA的结合。这些结果表明在自身免疫性疾病中存在与抗G抗体群体不同的自发产生的抗m7G抗体。