Taylor C M, Jones H C
Brain Res. 1985 May 13;334(1):27-31. doi: 10.1016/0006-8993(85)90563-3.
The cerebrospinal fluid (CSF) system of two amphibians, Rana catesbeiana and Rana pipiens has been perfused from the lateral ventricle to a site in the subarachnoid space close to the roof of the fourth ventricle. The CSF production rate (Vf) was measured by the dilution method using a perfusion fluid containing [14C]dextran in artificial CSF. For Rana catesbeiana Vf was 1.43 microliter min-1 or 0.2 microliter min-1 mg choroid plexus-1 and for Rana pipiens Vf was 0.2 microliter min-1 or 0.1 microliter min-1 mg choroid plexus-1. These control values declined by 35% and 14% respectively in the second half of the experimental period. Ouabain (5 X 10(-5) M) in the perfusion fluid for the second half of the experiment resulted in a much greater reduction in Vf, viz. 65% for Rana catesbeiana and 71% for Rana pipiens. Acetazolamide (1 X 10(-4) M) in the perfusion fluid for the second half of the experiment gave no change in Vf values when compared with control 74.5 +/- 4.8 microliters.
对牛蛙和豹蛙这两种两栖动物的脑脊液（CSF）系统进行了灌注，从侧脑室灌注至蛛网膜下腔中靠近第四脑室顶部的一个部位。脑脊液生成速率（Vf）采用稀释法进行测量，所用灌注液为含有[14C]葡聚糖的人工脑脊液。牛蛙的Vf为1.43微升/分钟或0.2微升/分钟·毫克脉络丛-1，豹蛙的Vf为0.2微升/分钟或0.1微升/分钟·毫克脉络丛-1。在实验期的后半段，这些对照值分别下降了35%和14%。在实验后半段的灌注液中加入哇巴因（5×10(-5)M），导致Vf下降幅度更大，即牛蛙下降了65%，豹蛙下降了71%。在实验后半段的灌注液中加入乙酰唑胺（1×10(-4)M），与对照值74.5±4.8微升相比，Vf值没有变化。
