Baudrie V, Roullet J B, Goureau Y, Chaouloff F, Elghozi J L
Laboratoire de Pharmacologie, CNRS I 61670, Faculté de Médecine Necker-Enfants Malades, Paris, France.
Fundam Clin Pharmacol. 1990;4(3):269-74. doi: 10.1111/j.1472-8206.1990.tb00494.x.
The inulin dilution technique was used to determine the cerebrospinal fluid (CSF) production rate by means of a push-pull cannula implanted into a lateral ventricle. Artificial CSF containing trace amount of 3H-inulin was perfused for 2 h in conscious rats. 3H-inulin in the effluent reached a plateau level depending on the CSF production rate. The control lateroventricular CSF production was 0.98 microliters/min. Production was reduced to 0.34 microliters/min during a perfusion with acetazolamide (1 mM), a carbonic anhydrase inhibitor.
采用菊粉稀释技术，通过植入侧脑室的推挽式套管来测定脑脊液（CSF）生成速率。在清醒大鼠中，灌注含微量3H-菊粉的人工脑脊液2小时。流出液中的3H-菊粉根据CSF生成速率达到平台期水平。对照组侧脑室CSF生成速率为0.98微升/分钟。在用碳酸酐酶抑制剂乙酰唑胺（1 mM）灌注期间，生成速率降至0.34微升/分钟。
