Institute of Biomedical Engineering, College of Life Science, Qingdao University, Qingdao 266071, China.
College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
Sensors (Basel). 2024 Apr 27;24(9):2787. doi: 10.3390/s24092787.
An abnormal level of dopamine (DA), a kind of neurotransmitter, correlates with a series of diseases, including Parkinson's disease, Willis-Ekbom disease, attention deficit hyperactivity disorder, and schizophrenia. Hence, it is imperative to achieve a precise, rapid detection method in clinical medicine. In this study, we synthesized nanocomposite carbon aerogels (CAs) doped with iron and iron carbide, based on algae residue-derived biomass materials, using Fe(NO) as the iron source. The modified glassy carbon electrode (GCE) for DA detection, denoted as CAs-Fe/GCE, was prepared through surface modification with this composite material. X-ray photoelectron spectroscopy and X-ray diffraction characterization confirmed the successful doping of iron into the as-prepared CAs. Additionally, the electrochemical behavior of DA on the modified electrode surface was investigated and the results demonstrate that the addition of the CAs-Fe promoted the electron transfer rate, thereby enhancing their sensing performance. The fabricated electrochemical DA biosensor exhibits an accurate detection of DA in the concentration within the range of 0.01~200 µM, with a detection limit of 0.0033 µM. Furthermore, the proposed biosensor is validated in real samples, showing its high applicability for the detection of DA in beverages.
多巴胺（DA）是一种神经递质，其水平异常与一系列疾病相关，包括帕金森病、不宁腿综合征、注意缺陷多动障碍和精神分裂症等。因此，在临床医学中实现精确、快速的检测方法至关重要。在本研究中，我们使用 Fe(NO)作为铁源，基于藻类残渣衍生的生物质材料合成了掺杂铁和碳化铁的纳米复合碳气凝胶（CAs）。通过用这种复合材料对修饰后的玻碳电极（GCE）进行表面修饰，制备了用于 DA 检测的修饰玻碳电极（CAs-Fe/GCE）。X 射线光电子能谱和 X 射线衍射表征证实了铁成功掺杂到所制备的 CAs 中。此外，还研究了修饰电极表面上 DA 的电化学行为，结果表明 CAs-Fe 的添加促进了电子转移速率，从而提高了它们的传感性能。所构建的电化学 DA 生物传感器在 0.01~200 µM 的浓度范围内对 DA 进行了准确检测，检测限为 0.0033 µM。此外，该生物传感器在实际样品中得到了验证，表明其在检测饮料中的 DA 方面具有很高的适用性。
