State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, Fujian Agriculture and Forestry University, Fuzhou, China.
Joint Laboratory of Guangdong province and Hong Kong region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, China.
J Sci Food Agric. 2024 Sep;104(12):7464-7475. doi: 10.1002/jsfa.13566. Epub 2024 May 10.
Dietary kelp possesses a variety of useful biological qualities but does not have a toxic effect on the host. In this study, we examine how kelp dietary supplementation enhances the serum biochemistry, intestinal immunity, and metabolism of hybrid snakehead. A total of 810 juvenile hybrid snakeheads (Channa maculata ♀ × Channa argus ♂), with an initial average weight of 11.4 ± 0.15 g, were allocated randomly to three treatment groups (three replicates per group). The fish were fed for 60 days with isonitrogenous and isolipidic diets. The groups were the control group (C) (20% high-gluten flour), the medium replacement group (MR) (10% high-gluten flour and 10% kelp meal), and the full replacement group (FR) (0% high-gluten flour and 15% kelp meal).
The results showed that dietary kelp increased the activity of serum antioxidant enzymes significantly and decreased the content of serum malondialdehyde (MDA) in hybrid snakeheads, with significant changes in the FR group (P < 0.05). The intestinal morphology results showed that dietary kelp helped to increase the specific surface area of intestinal villi, which was beneficial for intestinal digestion and absorption. According to transcriptome and quantitative real-time polymerase chain reaction (qRT-PCR) analysis, dietary kelp can improve the expression of intestinal immunity and metabolism-related pathways. Among them, immune-related genes MHC1 and HSPA1 were significantly up-regulated, and IGH, MHC2, and IL-8 were significantly down-regulated (P < 0.05). Lipid metabolism-related genes DGAT2, FABP2, RXRα, and PLPP1 were all significantly up-regulated (P < 0.05).
Dietary kelp can effectively improve the antioxidant function of hybrid snakeheads, improve intestinal morphology, reduce intestinal inflammation, and promote intestinal lipid synthesis and transportation, thereby improving intestinal immunity and metabolic functions. © 2024 Society of Chemical Industry.
裙带菜具有多种有益的生物学特性，对宿主无毒副作用。本研究探讨了裙带菜饲料补充如何增强杂交乌鳢的血清生化、肠道免疫和代谢。总共 810 尾平均初始体重为 11.4±0.15g 的杂交乌鳢幼鱼（♀ Channa maculata ×♂ Channa argus）被随机分配到三个处理组（每组三个重复）。鱼用等氮和等脂的饮食喂养 60 天。三个组分别为对照组（C）（20%高筋面粉）、中取代组（MR）（10%高筋面粉和 10%裙带菜粉）和全取代组（FR）（0%高筋面粉和 15%裙带菜粉）。
结果表明，饲料裙带菜显著提高了杂交乌鳢血清抗氧化酶的活性，降低了血清丙二醛（MDA）的含量，FR 组有显著变化（P<0.05）。肠道形态结果表明，饲料裙带菜有助于增加肠绒毛的比表面积，有利于肠道消化吸收。根据转录组和定量实时聚合酶链反应（qRT-PCR）分析，饲料裙带菜可以改善肠道免疫和代谢相关途径的表达。其中，免疫相关基因 MHC1 和 HSPA1 显著上调，IGH、MHC2 和 IL-8 显著下调（P<0.05）。脂质代谢相关基因 DGAT2、FABP2、RXRα 和 PLPP1 均显著上调（P<0.05）。
饲料裙带菜能有效提高杂交乌鳢的抗氧化功能，改善肠道形态，减轻肠道炎症，促进肠道脂质合成和转运，从而提高肠道免疫和代谢功能。© 2024 化学工业协会。
