University Joint Laboratory of Guangdong Province, Hong Kong and Macao Region on Marine Bioresource Conservation and Exploitation, Guangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 16 510070, China.
Fish Shellfish Immunol. 2023 Aug;139:108916. doi: 10.1016/j.fsi.2023.108916. Epub 2023 Jun 22.
To assess the level of oxidative stress, expression of immune-related genes, histomorphology, and changes in the intestinal tract of hybrid snakeheads(Channa maculata ♀ × Channa argus ♂) under stress from kelp powder in place of flour against Aeromonas hydrophila. We set up experimental diets: a control (C) diet of 20% flour, an experimental (MR) diet of 10% kelp powder and 10% flour, and an experimental (FR) diet of 0% starch and 15% kelp powder. The experimental fish in each group were infected with Aeromonas hydrophila after 60 days of feeding. For this experiment, some of the experimental fish in group C were injected with PBS as a negative control group (PBS). The results showed that the C group had significantly higher SOD, CAT, and T-AOC activity and expression of TAK1, IKKβ, IL-1β, and TNF-α genes in the MyD88 pathway than the PBS group. CAT activity and the expression of TAK1, IL-1β and TNF-α genes in the MyD88 pathway were significantly lower in the MR group than in the C group. Furthermore, the number of goblet cells in the MR group was significantly higher than in the C group. Furthermore, microorganisms such as Bacteroidota and Actinobacteriota were significantly lower in the C group than in the PBS and FR groups, as were beneficial bacteria such as Clostridium_sensu_stricto_1 and Sphingomonas. Replacing flour with kelp powder increases hybrid snakehead gut resistance to Aeromonas hydrophila.
为了评估氧化应激水平、免疫相关基因表达、组织形态学以及在以海带粉代替面粉的压力下杂交乌鳢（♀ Channa maculata ×♂ Channa argus）的肠道变化，以抵抗嗜水气单胞菌。我们设置了实验饮食：20%面粉的对照（C）饮食、10%海带粉和 10%面粉的实验（MR）饮食，以及 0%淀粉和 15%海带粉的实验（FR）饮食。每组实验鱼在喂养 60 天后感染嗜水气单胞菌。对于这项实验，C 组中的一些实验鱼被注射 PBS 作为阴性对照组（PBS）。结果表明，与 PBS 组相比，C 组的 SOD、CAT 和 T-AOC 活性以及 MyD88 通路中的 TAK1、IKKβ、IL-1β 和 TNF-α基因的表达显著升高。MR 组的 CAT 活性以及 MyD88 通路中的 TAK1、IL-1β 和 TNF-α基因的表达明显低于 C 组。此外，MR 组的杯状细胞数量明显高于 C 组。此外，与 PBS 和 FR 组相比，C 组的拟杆菌门和放线菌门等微生物以及厚壁菌门 sensu stricto 1 和 Sphingomonas 等有益菌明显减少。用海带粉代替面粉可提高杂交乌鳢对嗜水气单胞菌的肠道抵抗力。
