Ahadaf Soumaya, Azzouz Safae, Galiou Ouiam El, Errahmouni Mohamed Akram, Mentag Rachid, Arakrak Abdelhay, Laglaoui Amin
Biotechnology and Biomolecular Engineering Research Team, Department of Biology, Faculty of Science and Technology, Abdelmalek Essaâdi University, Tetouan, Morocco.
Biotechnology Research Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principale, 10090, Rabat, Morocco.
Probiotics Antimicrob Proteins. 2024 May 11. doi: 10.1007/s12602-024-10284-6.
Enterococcus, a common commensal organism in the human gut, exhibits a dual nature with certain strains offering probiotic benefits, while others are associated with nosocomial infections. In this study, we conducted a comprehensive examination of the genome of Enterococcus mundtii strain 203 to assess its probiotic potential and safety profile. The complete genome sequencing, assembly, and annotation were performed, followed by bioinformatics analysis. Our investigation reveals a detailed characterization of the Enterococcus mundtii 203 genome, originally isolated from camel feces, with a size of 3,053,234 bases and a GC content of 38.4%. Importantly, our analysis suggests that this strain poses no risk as a human pathogen due to the absence of antibiotic resistance determinants and virulence factors. The genome harbors a multitude of genes responsible for lactic acid production, bioactive peptide synthesis, adhesion molecule expression, resistance to harsh gut conditions, and enhancement of host metabolism. These findings underline the potential probiotic functionality of Enterococcus mundtii 203, positioning it as a promising candidate. Notably, our study did not identify any sequences related to insertion elements or CRISPR-Cas fragments.
肠球菌是人类肠道中常见的共生生物，具有双重特性，某些菌株具有益生菌益处，而其他菌株则与医院感染有关。在本研究中，我们对蒙氏肠球菌菌株203的基因组进行了全面检查，以评估其益生菌潜力和安全性。进行了全基因组测序、组装和注释，随后进行了生物信息学分析。我们的研究揭示了最初从骆驼粪便中分离出的蒙氏肠球菌203基因组的详细特征，其大小为3,053,234个碱基，GC含量为38.4%。重要的是，我们的分析表明，由于缺乏抗生素抗性决定簇和毒力因子，该菌株作为人类病原体没有风险。该基因组含有许多负责乳酸产生、生物活性肽合成、粘附分子表达、抵抗恶劣肠道条件以及增强宿主代谢的基因。这些发现强调了蒙氏肠球菌203潜在的益生菌功能，使其成为一个有前途的候选菌株。值得注意的是，我们的研究没有发现任何与插入元件或CRISPR-Cas片段相关的序列。
