Department of Cardiac Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050000, China.
Department of Cardiac Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050000, China.
Talanta. 2024 Aug 15;276:126228. doi: 10.1016/j.talanta.2024.126228. Epub 2024 May 7.
Multiplexed analysis of biomarkers in a single sample tube is essential for accurate diagnosis and therapy of diseases. However, the existing detection platforms suffer from many drawbacks, such as low specificity, limited applicable sceneries, and complicated operation. Hence, it is highly important to develop a versatile biomarker detection platform that can be used for disease diagnosis and pathophysiological research. In this study, we provide a versatile method for detecting biomarkers using dual-loop probes and quantum dots (QDs). This approach utilizes a dual-loop probe that consists of a recognition module for identifying specific targets, a template recognition module for initiating subsequent chain replacement cycles, and a signal module for facilitating the fixation of QDs on the 96-well plate. The lower limit of detection for miRNA-21 is determined to be at the aM level. Furthermore, this design may be easily expanded to simultaneously detect several targets, such as miRNA and C-reactive protein. The experimental results demonstrated the successful construction of the versatile biomarkers detection platform, and indicated that the sensitive and versatile platform has significant potential in the areas of bio-sensing, clinical diagnostics, and environmental sample analysis.
在单个样品管中进行多种生物标志物的分析对于疾病的准确诊断和治疗至关重要。然而，现有的检测平台存在许多缺点，例如特异性低、适用场景有限和操作复杂。因此，开发一种多功能的生物标志物检测平台，用于疾病诊断和病理生理研究非常重要。在本研究中，我们提供了一种使用双环探针和量子点（QDs）检测生物标志物的通用方法。该方法利用双环探针，它由用于识别特定靶标的识别模块、用于启动后续链替换循环的模板识别模块以及用于促进 QD 固定在 96 孔板上的信号模块组成。miRNA-21 的检测下限确定为 aM 级。此外，这种设计可以很容易地扩展到同时检测多个目标，如 miRNA 和 C-反应蛋白。实验结果证明了多功能生物标志物检测平台的成功构建，并表明该灵敏且多功能的平台在生物传感、临床诊断和环境样品分析等领域具有重要的应用潜力。
