Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Key Laboratory of Polyoxometalate Science of Ministry of Education, National & Local United Engineering Laboratory for Power Batteries, Department of Chemistry, Northeast Normal University, Changchun, Jilin Province, 130024, PR China.
Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Key Laboratory of Polyoxometalate Science of Ministry of Education, National & Local United Engineering Laboratory for Power Batteries, Department of Chemistry, Northeast Normal University, Changchun, Jilin Province, 130024, PR China.
Anal Chim Acta. 2020 Apr 8;1105:87-94. doi: 10.1016/j.aca.2020.01.034. Epub 2020 Jan 22.
Multi-target detection has been widely applied for the sensitive measurement of cancer-related biomarkers; however, the design and application of single platforms for diverse target detection are still challenging. Herein, a robust and sensitive electrochemiluminescence (ECL) biosensing platform was constructed for the measurement of microRNA-21 (miRNA-21) and mucin 1 (MUC1) based on dual catalytic hairpin assembly (DCHA). The catalytic hairpin assembly (CHA) process (Cycle I) was initiated by the target miRNA-21 to introduce abundant CdS:Mn quantum dots (CdS:Mn QDs) on the electrode surface, leading to a considerable ECL response and the sensitive detection of miRNA-21 with a limit of detection as low as 11 aM. Subsequently, the second CHA process (Cycle II) was triggered by the MUC1-aptamer complex, which allowed copious amounts of Au nanoparticles (AuNPs) to approach the CdS:Mn QDs. A decreased ECL signal was obtained due to the ECL resonance energy transfer (ECL-RET) effect between the CdS:Mn QDs and AuNPs; meanwhile, MUC1 was sensitively detected with a limit of detection of 0.40 fg mL. This single sensing platform achieved dual cancer-related biomarker detection, which could provide a rational approach for future clinical analyse.
多目标检测已广泛应用于癌症相关生物标志物的灵敏测量；然而，设计和应用单一平台进行多种目标检测仍然具有挑战性。在此，基于双催化发夹组装（DCHA）构建了一种用于测量 microRNA-21（miRNA-21）和粘蛋白 1（MUC1）的稳健且灵敏的电致化学发光（ECL）生物传感平台。催化发夹组装（CHA）过程（循环 I）由靶 miRNA-21 引发，在电极表面引入丰富的 CdS:Mn 量子点（CdS:Mn QDs），导致 ECL 响应显著增强，并对 miRNA-21 进行灵敏检测，检测限低至 11 aM。随后，通过 MUC1-适体复合物触发第二个 CHA 过程（循环 II），允许大量的金纳米粒子（AuNPs）接近 CdS:Mn QDs。由于 CdS:Mn QDs 和 AuNPs 之间的 ECL 共振能量转移（ECL-RET）效应，得到了一个降低的 ECL 信号；同时，MUC1 被灵敏地检测到，检测限为 0.40 fg mL。该单一传感平台实现了双重癌症相关生物标志物的检测，为未来的临床分析提供了一种合理的方法。
