Sustainable Development Study Centre, Government College University, Lahore 54000, Pakistan.
Department of Agriculture, Government College University, Lahore 54000, Pakistan; Tasmanian Institute of Agriculture, Mount Pleasant Laboratories, University of Tasmania, Launceston, Australia; School of Biological Sciences, University of Western Australia, Perth, Australia.
Plant Physiol Biochem. 2024 Jun;211:108711. doi: 10.1016/j.plaphy.2024.108711. Epub 2024 May 8.
Trace heavy metals (HMs) such as copper (Cu) and nickel (Ni) are toxic to plants, especially tomato at high levels. In this study, biochar (BC) was treated with amino acids (AA) to enhance amino functional groups, which effectively alleviated the adverse effects of heavy metals (HMs) on tomato growth. Hence, this study aimed to evaluate the effect of glycine and alanine modified BC (GBC/ABC) on various tomato growth parameters, its physiology, fruit yield and Cu/Ni uptake under Cu and Ni stresses. In a pot experiment, there was 21 treatments with three replications having two rates of simple BC and glycine/alanine enriched BC (0.5% and 1% (w/w). Cu and Ni stresses were added at 150 mg kg respectively. Plants were harvested after 120 days of sowing and subjected to various analysis. Under Cu and Ni stresses, tomato roots accumulated more Cu and Ni than shoots and fruits, while GBC and ABC application significantly enhanced the root and shoot dry weight irrelevant to the stress conditions. Both rates of GBC decreased the malondialdehyde and hydrogen peroxide levels in plants. The addition of 0.5% GBC with Cu enhanced the tomato fruit dry weight by 1.3 folds in comparison to the control treatment; while tomato fruit juice content also increased (50%) in the presence of 0.5% GBC with Ni as compared to control. In summary, these results demonstrated that lower rate of GBC∼0.5% proved to be the best in mitigating the Cu and Ni stress on tomato plant growth by enhancing the fruit production.
痕量重金属（HMs）如铜（Cu）和镍（Ni）对植物有毒，尤其是在高浓度下对番茄有毒。在这项研究中，生物炭（BC）用氨基酸（AA）处理以增强氨基官能团，这有效地减轻了重金属（HMs）对番茄生长的不利影响。因此，本研究旨在评估甘氨酸和丙氨酸修饰的生物炭（GBC/ABC）对各种番茄生长参数、其生理、果实产量和 Cu/Ni 吸收的影响，在 Cu 和 Ni 胁迫下。在盆栽试验中，有 21 种处理，其中 3 种重复有两种简单生物炭和富含甘氨酸/丙氨酸的生物炭（0.5%和 1%（w/w）。分别添加 150 mg kg 的 Cu 和 Ni 胁迫。播种后 120 天收获植物并进行各种分析。在 Cu 和 Ni 胁迫下，番茄根比茎叶和果实积累更多的 Cu 和 Ni，而 GBC 和 ABC 的应用显著增强了根和茎叶的干重，与胁迫条件无关。两种浓度的 GBC 均降低了植物体内的丙二醛和过氧化氢水平。与对照处理相比，添加 0.5% GBC 可使番茄果实干重增加 1.3 倍，而添加 0.5% GBC 与 Ni 共存时，番茄果汁含量也增加了（50%）。总之，这些结果表明，较低浓度的 GBC∼0.5%被证明是通过提高果实产量来缓解番茄植物生长的 Cu 和 Ni 胁迫的最佳方法。
