Laboratory of Nano- and Microencapsulation of Biologically Active Substances, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, St. Petersburg 195251, Russia; ITMO University, Kronverksky Prospekt 49, bldg. A, St. Petersburg 191002, Russia.
Laboratory of Nano- and Microencapsulation of Biologically Active Substances, Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, St. Petersburg 195251, Russia; St. Petersburg Academic University, St. Petersburg, Russia.
Nanomedicine. 2024 Jul;59:102753. doi: 10.1016/j.nano.2024.102753. Epub 2024 May 9.
In this study, we have considered four types of nanoparticles (NPs): polylactic acid (PLA), gold (Au), calcium carbonate (CaCO), and silica (SiO) with similar sizes (TEM: 50-110 nm and DLS: 110-140 nm) to examine their passive accumulation in three different tumors: colon (CT26), melanoma (B16-F10), and breast (4T1) cancers. Our results demonstrate that each tumor model showed a different accumulation of NPs, in the following order: CT26 > B16-F10 > 4T1. The Au and PLA NPs were evidently characterized by a higher delivery efficiency in case of CT26 tumors compared to CaCO and SiO NPs. The Au NPs demonstrated the highest accumulation in B16-F10 cells compared to other NPs. These results were verified using SPECT, ex vivo fluorescence bioimaging, direct radiometry and histological analysis. Thus, this work contributes to new knowledge in passive tumor targeting of NPs and can be used for the development of new strategies for delivery of bioactive compounds.
在这项研究中，我们考虑了四种类型的纳米颗粒（NPs）：聚乳酸（PLA）、金（Au）、碳酸钙（CaCO）和二氧化硅（SiO），它们具有相似的尺寸（TEM：50-110nm 和 DLS：110-140nm），以研究它们在三种不同肿瘤中的被动积累：结肠癌（CT26）、黑色素瘤（B16-F10）和乳腺癌（4T1）。我们的结果表明，每种肿瘤模型对 NPs 的积累都不同，顺序如下：CT26>B16-F10>4T1。与 CaCO 和 SiO NPs 相比，Au 和 PLA NPs 在 CT26 肿瘤中的递送效率显然更高。与其他 NPs 相比，Au NPs 在 B16-F10 细胞中的积累最高。这些结果通过 SPECT、离体荧光生物成像、直接辐射测量和组织学分析得到了验证。因此，这项工作为 NPs 的被动肿瘤靶向提供了新知识，并可用于开发生物活性化合物传递的新策略。
