Iaffaioli R V, Maio M, Ruggiero G, De Felice M, Ungaro A, Del Vecchio L, Rosato G B, Bianco A R, Zappacosta S
Int J Cancer. 1985 May 15;35(5):581-5. doi: 10.1002/ijc.2910350503.
A group of 157 women with primary breast cancer (BC) were typed for HLA antigens, and gene frequencies were compared to those of 327 control healthy individuals. Diagnosis of BC was made for all patients on surgical mastectomy specimens; histologic grading, estrogen (ER) and progesterone (PgR) receptors were determined on all primary tumors. Typed antigens included the majority of the specificities controlled by the HLA-A, -B and -C loci, according to the 8th International Histocompatibility Testing Workshop recommendations. No significant discrepancy in their frequencies was found in the undivided sample as compared to controls. The analysis of HLA gene frequency was extended to subsets of patients identified by the following prognostic features: (a) age at tumor diagnosis (pre-menopause vs. post-menopause); (b) receptor status (presence vs. absence of ER and PgR); (c) mammary gland dysplasia (presence vs. absence); (d) histologic grade (grade 3 vs. grades 1 and 2 combined); (e) time to relapse before or after 24 months following mastectomy). A moderate deviation from normal of some genes was found in several subsets, often affecting only one of the antithetical subgroups (feature present vs. feature absent). In the instance of B5, the increase in frequency of the gene in one of the subset pair (ER + subjects) was balanced by a decrease of the same gene in the counterpart (ER-subjects). Increased frequencies were found for the B7 gene in the following prognostic groups: (a) lack of ER (0.08); (b) lack of PgR (0.09); (c) absence of mammary dysplasia (0.075); (d) histologic grade 3 (0.10); and (e) premenopause (0.12), the last two showing significant divergence from normal. When features (d) and (e) on the one hand and (a), (b), (d) and (e) on the other were combined, B7 reached frequencies of 0.18 (p less than 5 X 10(-4] and of 0.29 (p less than 5 X 10(-6], respectively.
对157名原发性乳腺癌（BC）女性进行了HLA抗原分型，并将基因频率与327名健康对照个体的基因频率进行了比较。所有患者均通过手术切除的乳房标本确诊为BC；对所有原发性肿瘤进行了组织学分级、雌激素（ER）和孕激素（PgR）受体检测。根据第八届国际组织相容性检测研讨会的建议，分型抗原包括由HLA - A、- B和 - C位点控制的大多数特异性。与对照组相比，在未分组的样本中未发现其频率有显著差异。HLA基因频率分析扩展到根据以下预后特征确定的患者亚组：（a）肿瘤诊断时的年龄（绝经前与绝经后）；（b）受体状态（ER和PgR的存在与否）；（c）乳腺发育异常（存在与否）；（d）组织学分级（3级与1级和2级合并）；（e）乳房切除术后24个月之前或之后的复发时间）。在几个亚组中发现一些基因与正常情况有中度偏差，通常仅影响其中一个对偶亚组（特征存在与特征不存在）。以B5为例，该基因在其中一个亚组对（ER + 受试者）中的频率增加，被对应亚组（ER - 受试者）中相同基因的频率降低所平衡。在以下预后组中发现B7基因频率增加：（a）ER缺乏（0.08）；（b）PgR缺乏（0.09）；（c）无乳腺发育异常（0.075）；（d）组织学3级（0.10）；以及（e）绝经前（0.12），最后两项与正常情况有显著差异。当一方面将特征（d）和（e）与另一方面的（a）、（b）、（d）和（e）组合时，B7的频率分别达到0.18（p小于5×10^(-4)）和0.29（p小于5×10^(-6)）。
