Rogers T J, DeHaven J I, Donnelly R P
Int J Immunopharmacol. 1985;7(1):153-6. doi: 10.1016/0192-0561(85)90020-7.
Previous studies have shown that the prostaglandin-induced T-cell-derived suppressors (PITS) are actually a mixture of at least seven distinct factors. These factors may be reproducibly resolved by size-exclusion chromatography followed by high-pressure liquid chromatography (HPLC). Results are presented here which suggest that two T-lymphocyte hybridoma clones are capable of constitutive synthesis of three of these suppressor factors. Results show that clone Hyb-7SC2 produces factors which co-elute (by HPLC) with the PITS beta2 and PITS beta3 factors, while clone Hyb-9SC2 produces a factor which co-elutes with PITS beta1. These results suggest the possibility that the seven PITS beta factors are not the product of a single cell population.
先前的研究表明，前列腺素诱导的T细胞衍生抑制因子（PITS）实际上是至少七种不同因子的混合物。这些因子可以通过尺寸排阻色谱法，然后进行高压液相色谱法（HPLC）可重复地分离。此处给出的结果表明，两个T淋巴细胞杂交瘤克隆能够组成性合成其中三种抑制因子。结果显示，克隆Hyb-7SC2产生的因子与PITS β2和PITS β3因子（通过HPLC）共洗脱，而克隆Hyb-9SC2产生的一种因子与PITS β1共洗脱。这些结果提示了七种PITS β因子并非单一细胞群体产物的可能性。
