Nawata Y, Koike T, Hosokawa H, Tomioka H, Yoshida S
J Immunol. 1985 Jul;135(1):478-82.
The anti-IgE autoantibody in the IgG class was detected in 39/45 (86.7%) patients with atopic dermatitis by using a newly established solid-phase enzyme immunoassay. The epsilon-chain specificity of the anti-IgE autoantibody was confirmed by competitive inhibition by using human IgG, IgA, IgM, IgD, IgE, and heat-denatured IgE protein. Significant correlations were observed between the levels of the anti-IgE autoantibody and the serum IgE. Gel filtration studies indicated that the anti-IgE autoantibody in sera from atopic dermatitis was mainly present in the form of an immune complex with self IgE. The role of the IgE-anti-IgE immune complex and the role of the anti-IgE autoantibody in the modulation of the IgE-mediated immune system in atopic dermatitis are discussed.
采用新建立的固相酶免疫分析法，在45例特应性皮炎患者中的39例（86.7%）检测到了IgG类抗IgE自身抗体。通过用人IgG、IgA、IgM、IgD、IgE和热变性IgE蛋白进行竞争抑制，证实了抗IgE自身抗体的ε链特异性。观察到抗IgE自身抗体水平与血清IgE之间存在显著相关性。凝胶过滤研究表明，特应性皮炎患者血清中的抗IgE自身抗体主要以与自身IgE形成免疫复合物的形式存在。本文讨论了IgE - 抗IgE免疫复合物的作用以及抗IgE自身抗体在特应性皮炎中IgE介导的免疫系统调节中的作用。
