正常免疫反应过程中自身抗独特型抗体的产生。十二、自身抗独特型抗体的酶联免疫吸附测定法。
Production of auto-anti-idiotypic antibody during the normal immune response. XII. An enzyme-linked immunosorbent assay for auto-anti-idiotype antibody.
作者信息
Gibbons J J, Goidl E A, Shepherd G M, Thorbecke G J, Siskind G W
出版信息
J Immunol Methods. 1985 May 23;79(2):231-7. doi: 10.1016/0022-1759(85)90103-6.
PMID:3873501
Abstract
An enzyme-linked immunosorbent assay (ELISA) to detect anti-idiotype (Id) antibody is described. Using this assay auto-anti-Id was detected in the serum of aged mice immunized with 2,4,6-trinitrophenylated-Ficoll (TNP-F). Hapten eluates from anti-TNP-F immune spleen cells also contained readily detectable auto-anti-Id.
摘要
描述了一种用于检测抗独特型（Id）抗体的酶联免疫吸附测定（ELISA）。使用该测定法，在经2,4,6-三硝基苯化-聚蔗糖（TNP-F）免疫的老年小鼠血清中检测到了自身抗Id。来自抗TNP-F免疫脾细胞的半抗原洗脱液中也含有易于检测到的自身抗Id。
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