College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China.
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China.
Mar Genomics. 2024 Jun;75:101108. doi: 10.1016/j.margen.2024.101108. Epub 2024 Mar 24.
Dimethylsulfoniopropionate (DMSP) is one of the most abundant sulfur-containing organic compounds on the earth, which is an important carbon and sulfur source and plays an important role in the global sulfur cycle. Marine microorganisms are an important group involved in DMSP metabolism. The strain Cobetia sp. D5 was isolated from seawater samples in the Yellow Sea area of Qingdao during an algal bloom. There is still limited knowledge on the capacity of DMSP utilization of Cobetia bacteria. The study reports the whole genome sequence of Cobetia sp. D5 to understand its DMSP metabolism pathway. The genome of Cobetia sp. D5 consists of a circular chromosome with a length of 4,233,985 bp and the GC content is 62.56%. Genomic analysis showed that Cobetia sp. D5 contains a set of genes to transport and metabolize DMSP, which can cleave DMSP to produce dimethyl sulphide (DMS) and 3-Hydroxypropionyl-Coenzyme A (3-HP-CoA). DMS diffuses into the environment to enter the global sulfur cycle, whereas 3-HP-CoA is catabolized to acetyl CoA to enter central carbon metabolism. Thus, this study provides genetic insights into the DMSP metabolic processes of Cobetia sp. D5 during a marine algal bloom, and contributes to the understanding of the important role played by marine bacteria in the global sulfur cycle.
二甲基巯基丙酸酯（DMSP）是地球上最丰富的含硫有机化合物之一，是重要的碳源和硫源，在全球硫循环中发挥着重要作用。海洋微生物是参与 DMSP 代谢的重要群体。Cobetia sp. D5 菌株是从青岛黄海海域藻类大量繁殖期间的海水样本中分离出来的。目前对于 Cobetia 细菌利用 DMSP 的能力知之甚少。本研究报告了 Cobetia sp. D5 的全基因组序列，以了解其 DMSP 代谢途径。Cobetia sp. D5 的基因组由一条长度为 4,233,985bp 的环形染色体组成，GC 含量为 62.56%。基因组分析表明，Cobetia sp. D5 含有一套用于运输和代谢 DMSP 的基因，可将 DMSP 切割产生二甲硫（DMS）和 3-羟基丙酰辅酶 A（3-HP-CoA）。DMS 扩散到环境中进入全球硫循环，而 3-HP-CoA 则被分解为乙酰辅酶 A 进入中心碳代谢。因此，本研究为海洋藻类大量繁殖期间 Cobetia sp. D5 的 DMSP 代谢过程提供了遗传见解，并有助于理解海洋细菌在全球硫循环中所起的重要作用。
