Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey.
Department of Urology, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkey.
Rev Int Androl. 2024 Mar;22(1):1-7. doi: 10.22514/j.androl.2024.001. Epub 2024 Mar 30.
It was aimed to evaluate whether gallic acid (GA) have a beneficial effect in the testicular ischemia/reperfusion injury (IRI) model in rats for the first time. Testicular malondialdehyde, 8-hydroxy-2'-deoxyguanosine, superoxide dismutase, catalase, high mobility group box 1 protein, nuclear factor kappa B, tumor necrosis factoralpha, interleukin-6, myeloperoxidase, 78-kDa glucose-regulated protein, activating transcription factor 6, CCAAT-enhancer-binding protein homologous protein and caspase-3 levels were determined using colorimetric methods. The oxidative stress, inflammation, endoplasmic reticulum stress and apoptosis levels increased statistically significantly in the IRI group compared with the sham operated group ( < 0.05). GA application improved these damage significantly ( < 0.05). Moreover, it was found that the results of histological examinations supported the biochemical results to a statistically significant extent. Our findings suggested that GA may be evaluated as a protective agent against testicular IRI.
首次评估没食子酸（GA）对大鼠睾丸缺血/再灌注损伤（IRI）模型是否具有有益作用。采用比色法测定睾丸丙二醛、8-羟基-2'-脱氧鸟苷、超氧化物歧化酶、过氧化氢酶、高迁移率族蛋白 1 （HMGB1）、核因子κB（NF-κB）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、髓过氧化物酶（MPO）、78kDa 葡萄糖调节蛋白（GRP78）、激活转录因子 6（ATF6）、CCAAT 增强子结合蛋白同源蛋白（CHOP）和半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）水平。与假手术组相比，IRI 组的氧化应激、炎症、内质网应激和细胞凋亡水平显著升高（<0.05）。GA 应用显著改善了这些损伤（<0.05）。此外，组织学检查结果发现，这些结果在统计学上与生化结果具有显著的一致性。我们的研究结果表明，GA 可被评估为一种针对睾丸 IRI 的保护剂。
