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对 LACS 基因家族的全基因组表达分析表明，GhLACS25 基因在棉花中对盐胁迫具有功能响应。

Genome-wide expression analysis of LACS gene family implies GhLACS25 functional responding to salt stress in cotton.

机构信息

Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology, Anyang, Henan, 455000, China.

Henan University/State Key Laboratory of Crop Stress Adaptation and Improvement, Kaifeng, Henan, 475004, China.

出版信息

BMC Plant Biol. 2024 May 13;24(1):392. doi: 10.1186/s12870-024-05045-0.

DOI:10.1186/s12870-024-05045-0
PMID:38735932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11089787/
Abstract

BACKGROUND

Long-chain acyl-coenzyme A synthetase (LACS) is a type of acylating enzyme with AMP-binding, playing an important role in the growth, development, and stress response processes of plants.

RESULTS

The research team identified different numbers of LACS in four cotton species (Gossypium hirsutum, Gossypium barbadense, Gossypium raimondii, and Gossypium arboreum). By analyzing the structure and evolutionary characteristics of the LACS, the GhLACS were divided into six subgroups, and a chromosome distribution map of the family members was drawn, providing a basis for further research classification and positioning. Promoter cis-acting element analysis showed that most GhLACS contain plant hormones (GA, MeJA) or non-biological stress-related cis-elements. The expression patterns of GhLACS under salt stress treatment were analyzed, and the results showed that GhLACS may significantly participate in salt stress response through different mechanisms. The research team selected 12 GhLACSs responsive to salt stress for tissue expression analysis and found that these genes are expressed in different tissues.

CONCLUSIONS

There is a certain diversity of LACS among different cotton species. Analysis of promoter cis-acting elements suggests that GhLACS may be involved in regulating plant growth, development and stress response processes. GhLACS25 was selected for in-depth study, which confirmed its significant role in salt stress response through virus-induced gene silencing (VIGS) and induced expression in yeast cells.

摘要

背景

长链酰基辅酶 A 合成酶（LACS）是一种具有 AMP 结合的酰化酶，在植物的生长、发育和应激响应过程中发挥着重要作用。

结果

研究团队在四个棉花物种（陆地棉、海岛棉、亚洲棉和草棉）中鉴定出不同数量的 LACS。通过分析 LACS 的结构和进化特征，将 GhLACS 分为六个亚组，并绘制了家族成员的染色体分布图，为进一步的研究分类和定位提供了依据。启动子顺式作用元件分析表明，大多数 GhLACS 含有植物激素（GA、MeJA）或非生物胁迫相关的顺式元件。分析了 GhLACS 在盐胁迫处理下的表达模式，结果表明 GhLACS 可能通过不同的机制显著参与盐胁迫响应。研究团队选择了 12 个对盐胁迫响应的 GhLACS 进行组织表达分析，发现这些基因在不同的组织中表达。

结论

不同棉花物种之间的 LACS 存在一定的多样性。启动子顺式作用元件分析表明，GhLACS 可能参与调节植物的生长、发育和应激响应过程。选择 GhLACS25 进行深入研究，通过病毒诱导的基因沉默（VIGS）和酵母细胞中的诱导表达证实了其在盐胁迫响应中的重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/75e7d47218c2/12870_2024_5045_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/72ca972b189a/12870_2024_5045_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/925c373893b9/12870_2024_5045_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/01ae475bef84/12870_2024_5045_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/886555201574/12870_2024_5045_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/43e8795350b2/12870_2024_5045_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/839bd7efd268/12870_2024_5045_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/f51f36451469/12870_2024_5045_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/787daac3e409/12870_2024_5045_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/75e7d47218c2/12870_2024_5045_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/72ca972b189a/12870_2024_5045_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/925c373893b9/12870_2024_5045_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/01ae475bef84/12870_2024_5045_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/886555201574/12870_2024_5045_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/43e8795350b2/12870_2024_5045_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/839bd7efd268/12870_2024_5045_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/f51f36451469/12870_2024_5045_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/787daac3e409/12870_2024_5045_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bcc/11089787/75e7d47218c2/12870_2024_5045_Fig9_HTML.jpg

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