National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, 455000, China.
Zhucheng Agricultural Technology Promotion Center, Zhucheng, Shandong, 262200, China.
BMC Plant Biol. 2023 Dec 19;23(1):653. doi: 10.1186/s12870-023-04657-2.
Cotton, being extensively cultivated, holds immense economic significance as one of the most prominent crops globally. The SET (Su(var), E, and Trithorax) domain-containing protein is of significant importance in plant development, growth, and response to abiotic stress by modifying the lysine methylation status of histone. However, the comprehensive identification of SET domain genes (SDG) have not been conducted in upland cotton (Gossypium hirsutum L.).
A total of 229 SDGs were identified in four Gossypium species, including G. arboretum, G. raimondii, G. hirsutum, and G. barbadense. These genes could distinctly be divided into eight groups. The analysis of gene structure and protein motif revealed a high degree of conservation among the SDGs within the same group. Collinearity analysis suggested that the SDGs of Gossypium species and most of the other selected plants were mainly expanded by dispersed duplication events and whole genome duplication (WGD) events. The allopolyploidization event also has a significant impact on the expansion of SDGs in tetraploid Gossypium species. Furthermore, the characteristics of these genes have been relatively conserved during the evolution. Cis-element analysis revealed that GhSDGs play a role in resistance to abiotic stresses and growth development. Furthermore, the qRT-PCR results have indicated the ability of GhSDGs to respond to salt stress. Co-expression analysis revealed that GhSDG51 might co-express with genes associated with salt stress. In addition, the silencing of GhSDG51 in cotton by the virus-induced gene silencing (VIGS) method suggested a potential positive regulatory role of GhSDG51 in salt stress.
The results of this study comprehensively analyze the SDGs in cotton and provide a basis for understanding the biological role of SDGs in the stress resistance in upland cotton.
棉花作为一种广泛种植的作物，具有巨大的经济意义，是全球最重要的作物之一。SET（Su(var)、E 和 Trithorax）结构域蛋白在植物发育、生长和对非生物胁迫的反应中具有重要意义，通过修饰组蛋白的赖氨酸甲基化状态。然而，在陆地棉（Gossypium hirsutum L.）中尚未对 SET 结构域基因（SDG）进行全面鉴定。
在四个棉属物种（G. arboretum、G. raimondii、G. hirsutum 和 G. barbadense）中鉴定出了总共 229 个 SDG，这些基因可以明显分为八个组。基因结构和蛋白质基序分析表明，同一组内的 SDG 具有高度的保守性。共线性分析表明，棉属物种和大多数其他选定植物的 SDG 主要通过分散重复事件和全基因组重复（WGD）事件扩张。异源多倍化事件也对四倍体棉属物种 SDG 的扩张产生了重大影响。此外，这些基因在进化过程中具有相对保守的特征。顺式作用元件分析表明，GhSDG 参与了非生物胁迫和生长发育的抗性。此外，qRT-PCR 结果表明 GhSDG 能够响应盐胁迫。共表达分析表明，GhSDG51 可能与与盐胁迫相关的基因共表达。此外，通过病毒诱导的基因沉默（VIGS）方法沉默棉花中的 GhSDG51 表明 GhSDG51 在盐胁迫中可能具有正向调节作用。
本研究全面分析了棉花中的 SDG，为了解 SDG 在陆地棉抗逆性中的生物学作用提供了依据。
