School of Life Sciences, Nantong University, Nantong, Jiangsu, 226019, China.
Jiangsu Coastal Area Institute of Agricultural Sciences/Jiangsu Collaborative Innovation Center for Modern Crop Production, Yancheng, Jiangsu, 224002, China.
BMC Genomics. 2024 May 24;25(1):513. doi: 10.1186/s12864-024-10388-x.
Aldehyde dehydrogenases (ALDHs) are a family of enzymes that catalyze the oxidation of aldehyde molecules into the corresponding carboxylic acid, regulate the balance of aldehydes and protect plants from the poisoning caused by excessive accumulation of aldehydes; however, this gene family has rarely been studied in cotton.
In the present study, genome-wide identification was performed, and a total of 114 ALDH family members were found in three cotton species, Gossypium hirsutum, Gossypium arboreum and Gossypium raimondii. The ALDH genes were divided into six subgroups by evolutionary analysis. ALDH genes in the same subgroup showed similar gene structures and conserved motifs, but some genes showed significant differences, which may result in functional differences. Chromosomal location analysis and selective pressure analysis revealed that the ALDH gene family had experienced many fragment duplication events. Cis-acting element analysis revealed that this gene family may be involved in the response to various biotic and abiotic stresses. The RT‒qPCR results showed that the expression levels of some members of this gene family were significantly increased under salt stress conditions. Gohir.A11G040800 and Gohir.D06G046200 were subjected to virus-induced gene silencing (VIGS) experiments, and the sensitivity of the silenced plants to salt stress was significantly greater than that of the negative control plants, suggesting that Gohir.A11G040800 and Gohir.D06G046200 may be involved in the response of cotton to salt stress.
In total, 114 ALDH genes were identified in three Gossypium species by a series of bioinformatics analysis. Gene silencing of the ALDH genes of G. hirsutum revealed that ALDH plays an important role in the response of cotton to salt stress.
醛脱氢酶（ALDHs）是一类能够催化醛分子氧化为相应羧酸的酶，调节醛的平衡，保护植物免受醛类过量积累引起的毒害；然而，该基因家族在棉花中很少被研究。
本研究在三个棉种（Gossypium hirsutum、Gossypium arboreum 和 Gossypium raimondii）中进行了全基因组鉴定，共发现 114 个 ALDH 家族成员。通过进化分析，将 ALDH 基因分为六个亚组。同一亚组的 ALDH 基因具有相似的基因结构和保守基序，但有些基因存在显著差异，可能导致功能差异。染色体定位分析和选择压力分析表明，ALDH 基因家族经历了许多片段重复事件。顺式作用元件分析表明，该基因家族可能参与了对各种生物和非生物胁迫的响应。RT-qPCR 结果表明，该基因家族的一些成员在盐胁迫条件下表达水平显著升高。对 Gohir.A11G040800 和 Gohir.D06G046200 进行病毒诱导基因沉默（VIGS）实验，沉默植株对盐胁迫的敏感性明显高于阴性对照植株，表明 Gohir.A11G040800 和 Gohir.D06G046200 可能参与了棉花对盐胁迫的响应。
通过一系列生物信息学分析，在三个棉种中鉴定出 114 个 ALDH 基因。对 G. hirsutum 的 ALDH 基因进行基因沉默表明，ALDH 在棉花对盐胁迫的响应中发挥重要作用。
