Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Indian J Ophthalmol. 2024 Nov 1;72(Suppl 5):S801-S808. doi: 10.4103/IJO.IJO_3304_23. Epub 2024 May 13.
The gut microbiota might be closely related to central retinal artery occlusion (CRAO), but the causality has not been well defined. Two-sample Mendelian randomization (MR) study was used to reveal the potential causal effect between the gut microbiota and CRAO.
Data for gut microbiota were obtained from the genome-wide association studies of the Dutch Microbiome Project (DMP) ( n = 7738) and the MiBioGen consortium ( n = 18,340), and data on CRAO were obtained from samples of FinnGen project (546 cases and 344,569 controls). Causalities of exposures and outcomes were explored mainly using the inverse variance weighted method. In addition, multiple sensitivity analyses including MR-Egger, weighted median (WM), simple mode, weighted mode, and MR Pleiotropy RESidual Sum and Outlier were simultaneously applied to validate the final results.
We identified three microbial pathways (two risk factors/one protective factor) and seven microbial taxa (two risk factors/five protective factors) associated with CRAO in the DMP study. Based on the data from the MiBioGen consortium, we identified seven microbial taxa (two risk factors/five protective factors) associated with CRAO, including the Eubacterium genus, which was consistently identified as a risk factor in both the DMP and the MiBioGen consortium MR analyses.
Our study implicates the potential causal effects of specific microbial taxa and pathways on CRAO, potentially providing new insights into the prevention and treatment of CRAO through specific gut microbial taxa and pathway. Since our conclusion is a hypothesis derived from secondary genome-wide association studies (GWAS) data analysis, further research is needed for confirmation.
肠道微生物群与中央视网膜动脉阻塞（CRAO）可能密切相关，但因果关系尚未明确。本研究采用两样本孟德尔随机化（MR）研究来揭示肠道微生物群与 CRAO 之间的潜在因果关系。
肠道微生物群数据来自荷兰微生物组计划（DMP）（n=7738）和 MiBioGen 联盟的全基因组关联研究（n=18340），CRAO 数据来自 FinnGen 项目的样本（546 例和 344569 例对照）。主要采用逆方差加权法探索暴露和结局的因果关系。此外，同时应用 MR-Egger、加权中位数（WM）、简单模式、加权模式和 MR 偏倚 RESidual Sum and Outlier 等多种敏感性分析来验证最终结果。
我们在 DMP 研究中发现了与 CRAO 相关的三个微生物途径（两个风险因素/一个保护因素）和七个微生物分类群（两个风险因素/五个保护因素）。基于 MiBioGen 联盟的数据，我们确定了与 CRAO 相关的七个微生物分类群（两个风险因素/五个保护因素），包括真细菌属，该属在 DMP 和 MiBioGen 联盟的 MR 分析中均被一致鉴定为风险因素。
本研究提示了特定微生物分类群和途径对 CRAO 的潜在因果作用，可能为通过特定肠道微生物分类群和途径预防和治疗 CRAO 提供新的思路。由于我们的结论是基于二次全基因组关联研究（GWAS）数据分析得出的假设，因此需要进一步研究来证实。
