Jin Geonsoo, Upreti Neil, Rich Joseph, Xia Jianping, Zhao Chenglong, Huang Tony Jun
Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708 USA.
Department of Biomedical Engineering, Duke University, Durham, NC 27708 USA.
Microsyst Nanoeng. 2024 May 10;10:59. doi: 10.1038/s41378-024-00683-8. eCollection 2024.
Large-field nanoscale fluorescence imaging is invaluable for many applications, such as imaging subcellular structures, visualizing protein interactions, and high-resolution tissue imaging. Unfortunately, conventional fluorescence microscopy requires a trade-off between resolution and field of view due to the nature of the optics used to form the image. To overcome this barrier, we developed an acoustofluidic scanning fluorescence nanoscope that simultaneously achieves superior resolution, a large field of view, and strong fluorescent signals. The acoustofluidic scanning fluorescence nanoscope utilizes the superresolution capabilities of microspheres that are controlled by a programmable acoustofluidic device for rapid fluorescence enhancement and imaging. The acoustofluidic scanning fluorescence nanoscope resolves structures that cannot be resolved with conventional fluorescence microscopes with the same objective lens and enhances the fluorescent signal by a factor of ~5 without altering the field of view of the image. The improved resolution realized with enhanced fluorescent signals and the large field of view achieved acoustofluidic scanning fluorescence nanoscopy provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine, biology, biophysics, and biomedical engineering.
大视野纳米级荧光成像在许多应用中都具有重要价值，例如对亚细胞结构进行成像、观察蛋白质相互作用以及进行高分辨率组织成像。不幸的是，由于用于形成图像的光学器件的特性，传统荧光显微镜需要在分辨率和视野之间进行权衡。为了克服这一障碍，我们开发了一种声流体扫描荧光纳米显微镜，它能够同时实现卓越的分辨率、大视野和强荧光信号。声流体扫描荧光纳米显微镜利用微球的超分辨率能力，这些微球由可编程声流体装置控制，用于快速增强荧光并成像。声流体扫描荧光纳米显微镜能够分辨使用相同物镜的传统荧光显微镜无法分辨的结构，并且在不改变图像视野的情况下将荧光信号增强约5倍。声流体扫描荧光纳米显微镜实现的更高分辨率、增强的荧光信号以及大视野，为医学、生物学、生物物理学和生物医学工程领域的研究人员提供了一种用于通用纳米级荧光成像的强大工具。
