Younus Alaa H, Al-Faisal Abdul Hussein M
Biomedical Engineering Department, University of Technology, Iraq.
Institute of Genetic Engineering and Biotechnology, University of Baghdad, Baghdad, Iraq.
J Taibah Univ Med Sci. 2024 Apr 27;19(3):575-584. doi: 10.1016/j.jtumed.2024.04.005. eCollection 2024 Jun.
The transcription factor 7-like 2 gene () is associated with a predisposition to type 2 diabetes mellitus (T2DM) in different ethnic populations. This article investigated the relationship between gene expression and several biochemical indexes among different age categories of T2DM in a sample of the Iraqi population.
One hundred and fifty blood samples were collected from three groups: young T2DM (10-35 years), old T2DM (40-80 years), and healthy control (10-65 years) groups. Both sexes were enrolled. qPCR was performed to explore the expression of the gene. Biochemical tests were performed to assess hemoglobin A1C (HbA1c), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels. The body mass index (BMI) was calculated. The results were statistically analyzed.
Patients with T2DM had higher BMI, TG, and LDL, and lower HDL than the control group. There was a strong positive correlation between hemoglobin A1C (HbA1c) and BMI, TG, and LDL and a negative correlation between HbA1c and HDL. Expression of the gene showed a significant difference between old and young patients by 1.68 and 0.207 fold, respectively. These results showed that old patients had higher gene expression than young patients.
gene expression was affected by age, with higher expression in old T2DM patients. This may influence beta cell functions and insulin secretion.
转录因子7样2基因（）与不同种族人群患2型糖尿病（T2DM）的易感性相关。本文在伊拉克人群样本中研究了T2DM不同年龄组中该基因表达与几种生化指标之间的关系。
从三组人群中采集了150份血样：年轻T2DM组（10 - 35岁）、老年T2DM组（40 - 80岁）和健康对照组（10 - 65岁）。纳入了男女两性。采用qPCR检测该基因的表达。进行生化检测以评估糖化血红蛋白A1C（HbA1c）、甘油三酯（TG）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）水平。计算体重指数（BMI）。对结果进行统计学分析。
T2DM患者的BMI、TG和LDL高于对照组，HDL低于对照组。糖化血红蛋白A1C（HbA1c）与BMI、TG和LDL之间存在强正相关，与HDL之间存在负相关。该基因的表达在老年和年轻患者之间分别有1.68倍和0.207倍的显著差异。这些结果表明老年患者的基因表达高于年轻患者。
基因表达受年龄影响，老年T2DM患者中表达较高。这可能会影响β细胞功能和胰岛素分泌。
