Qiao Yongjing, Gong Jihong, Jin Ziqi, Tu Yiting, Yang Xiaofei
Key Laboratory of Cognitive Science, Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis & Treatment, Laboratory of Membrane Ion Channels and Medicine, College of Biomedical Engineering, South-Central Minzu University, Wuhan 430074, China.
Biophys Rep. 2024 Feb 29;10(1):41-47. doi: 10.52601/bpr.2023.230033.
Substrate stiffness is a microenvironment with a certain stiffness constructed by the extracellular matrix and adjacent cells, which plays an important role in the growth and development of cells and tissue formation. Studies have indicated that the stiffness of the brain is about 0.1-1 kPa. The physiological and pathological processes of the nervous system are mediated by the substrate stiffness that the neurons suffer. However, how substrate stiffness regulates these processes remains to be studied. Culturing neurons on substrates with different stiffness is one of the best methods to study the role of stiffness in regulating neuronal development and activity. In this study, by changing the preparation time and the activation time of polyacrylamide gel, we provide an improved method that achieves a low toxic substrate environment for better primary neuron adhesion and development. Hope that this method is convenient for those studying the role of substrate stiffness in neurons.
底物硬度是由细胞外基质和相邻细胞构建的具有一定硬度的微环境，其在细胞的生长发育和组织形成中发挥着重要作用。研究表明，大脑的硬度约为0.1-1千帕。神经系统的生理和病理过程由神经元所承受的底物硬度介导。然而，底物硬度如何调节这些过程仍有待研究。在具有不同硬度的底物上培养神经元是研究硬度在调节神经元发育和活动中作用的最佳方法之一。在本研究中，通过改变聚丙烯酰胺凝胶的制备时间和激活时间，我们提供了一种改进的方法，该方法实现了低毒性的底物环境，以利于原代神经元更好地黏附和发育。希望该方法对那些研究底物硬度在神经元中作用的人有所帮助。
