Liu Meiqi, Wang Zhen, Qin Chen, Cao Huiyan, Kong Lingyang, Liu Tingxia, Jiang Shan, Ma Lengleng, Liu Xiubo, Ren Weichao, Ma Wei
College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi 154002, China.
J Agric Food Chem. 2024 May 22;72(20):11429-11437. doi: 10.1021/acs.jafc.3c09293. Epub 2024 May 13.
is a medicinal plant whose main component is platycodins, which have a variety of pharmacological effects and nutritional values. The farnesyl pyrophosphate synthase (FPS) is a key enzyme in the isoprenoid biosynthesis pathway, which catalyzes the synthesis of farnesyl diphosphate (FPP). In this study, we cloned the gene from () with an ORF of 1260 bp, encoding 419 amino acids with a deduced molecular weight and theoretical pI of 46,200.98 Da and 6.52, respectively. The squalene content of overexpressed in tobacco leaves and yeast cells extract was 1.88-fold and 1.21-fold higher than that of the control group, respectively, and the total saponin content was also increased by 1.15 times in yeast cells extract, which verified the biological function of in terpenoid synthesis. After 48 h of MeJA treatment and 6 h of ethephon treatment, the expression of the gene in roots and stems reached its peak, showing a 3.125-fold and 3.236-fold increase compared to the untreated group, respectively. Interestingly, the expression of the gene in leaves showed a decreasing trend after exogenous elicitors treatment. The discovery of this enzyme will provide a novel perspective for enhancing the efficient synthesis of platycodins.
是一种药用植物，其主要成分是桔梗皂苷，具有多种药理作用和营养价值。法尼基焦磷酸合酶（FPS）是类异戊二烯生物合成途径中的关键酶，催化法尼基二磷酸（FPP）的合成。在本研究中，我们从（）克隆了该基因，其开放阅读框为1260 bp，编码419个氨基酸，推导的分子量和理论等电点分别为46,200.98 Da和6.52。烟草叶片和酵母细胞提取物中过表达的该基因的角鲨烯含量分别比对照组高1.88倍和1.21倍，酵母细胞提取物中的总皂苷含量也增加了1.15倍，这验证了该基因在萜类合成中的生物学功能。在茉莉酸甲酯（MeJA）处理48小时和乙烯利处理6小时后，该基因在根和茎中的表达达到峰值，与未处理组相比分别增加了3.125倍和3.236倍。有趣的是，在外源激发子处理后，该基因在叶片中的表达呈下降趋势。这种酶的发现将为提高桔梗皂苷的高效合成提供新的视角。
