Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, China.
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, China.
Arch Microbiol. 2024 May 13;206(6):259. doi: 10.1007/s00203-024-03988-4.
Nucleotides are important components and the main indicators for judging Cordyceps quality. In this paper, the mixed fermentation process of Schisandra chinensis and Cordyceps tenuipes was systematically studied, and it was proposed that the fermentation products aqueous extract (S-ZAE) had antioxidant activity and anti-AChE ability. Herein, the results of a single factor showed that S. chinensis, yeast extract, inoculum amount, and pH had significant effects on nucleotide synthesis. The fermentation process optimization results were 3% glucose, 0.25% KHPO, 2.1% yeast extract, and S. chinensis 0.49% (m/v), the optimal fermentation conditions were 25℃, inoculum 5.8% (v/v), pH 3.8, 6 d. The yield of total nucleotides in the scale-up culture was 0.64 ± 0.027 mg/mL, which was 10.6 times higher than before optimization. S-ZAE has good antioxidant and anti-AChE activities (IC 0.50 ± 0.050 mg/mL). This fermentation method has the advantage of industrialization, and its fermentation products have the potential to become good functional foods or natural therapeutic agents.
核苷酸是判断虫草质量的重要成分和主要指标。本文系统研究了五味子与蛹虫草的混合发酵工艺，提出发酵产物水提物（S-ZAE）具有抗氧化和抗 AChE 活性。单因素结果表明，五味子、酵母膏、接种量和 pH 值对核苷酸合成有显著影响。发酵过程优化结果为 3%葡萄糖、0.25%KHPO、2.1%酵母膏和 0.49%五味子（m/v），最佳发酵条件为 25℃、接种量 5.8%（v/v）、pH3.8、6d。放大培养的总核苷酸产量为 0.64±0.027mg/mL，比优化前提高了 10.6 倍。S-ZAE 具有良好的抗氧化和抗 AChE 活性（IC 0.50±0.050mg/mL）。该发酵方法具有工业化优势，其发酵产物具有成为良好的功能性食品或天然治疗剂的潜力。
