一种抗DNA单克隆抗体及其与DNA复合物的体内清除率和组织摄取情况。
In vivo clearance and tissue uptake of an anti-DNA monoclonal antibody and its complexes with DNA.
作者信息
Ben Chetrit E, Dunsky E H, Wollner S, Eilat D
出版信息
Clin Exp Immunol. 1985 Apr;60(1):159-68.
In vivo clearance and tissue localization of a purified mouse anti-DNA monoclonal antibody (MoAb) (A52 IgG2b) and its complexes with DNA were studied in normal BALB/c and autoimmune NZB/NZW mice. The plasma half-life of the autoantibody in both mouse strains was significantly shorter (T 1/2 = 10-15 min), compared with that of purified NZB myeloma proteins (T 1/2 greater than or equal to 180 min). DNA antigen and DNA-A52 IgG complexes in antibody excess were cleared very rapidly (T 1/2 = 4-8 min), while complexes formed in antigen excess persisted in the circulation much longer (T 1/2 = 60 min). Organ studies showed that the anti-DNA MoAb was transiently retained by the liver and the spleen but demonstrated a particular affinity for the kidney tissue. We suggest that tissue damage in SLE glomerulonephritis may be facilitated by direct interaction of anti-DNA antibodies with glomerular components.
在正常BALB/c小鼠和自身免疫性NZB/NZW小鼠中研究了纯化的小鼠抗DNA单克隆抗体（MoAb）（A52 IgG2b）及其与DNA的复合物在体内的清除和组织定位。与纯化的NZB骨髓瘤蛋白（T1/2大于或等于180分钟）相比，两种小鼠品系中自身抗体的血浆半衰期均显著缩短（T1/2 = 10 - 15分钟）。抗体过量时的DNA抗原和DNA - A52 IgG复合物清除非常迅速（T1/2 = 4 - 8分钟），而抗原过量时形成的复合物在循环中持续的时间长得多（T1/2 = 60分钟）。器官研究表明，抗DNA MoAb被肝脏和脾脏短暂保留，但对肾脏组织表现出特殊亲和力。我们认为，抗DNA抗体与肾小球成分的直接相互作用可能促进系统性红斑狼疮性肾小球肾炎中的组织损伤。
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