De Nardin E, Tanigaki N
Hybridoma. 1985 Summer;4(2):125-41. doi: 10.1089/hyb.1985.4.125.
An IgM monoclonal antibody directed against the T-cell E-rosette receptor was obtained by preparing a hybridoma against cells of a human T-cell leukemia line, HPB-ALL. The antibody, named EDN-34B1, reacts exclusively with cells of T-cell lineage, including those of three T-cell leukemia lines. It does not react with cells of B-cell lines, normal B-cells, or monocytes. EDN-34B1 recognizes a single polypeptide of approximately 50,000 molecular weight, is capable of blocking E-rosette formation, and its binding to the target cell can be specifically blocked by monoclonal antibodies 9.6 and OKT-11, both anti-E-receptor antibodies. EDN-34B1 is 100% cytotoxic against normal thymocytes and peripheral T-cells, thus enabling the routine removal of such cells from a mixed lymphocyte population, and kills a higher percentage of normal T-lymphocytes than Ab 9.6, even though immunofluorescence studies have shown that both antibodies bind to the same fraction of PBLs. This phenomenon may be due to the IgM nature of EDN-34B1. Addition of EDN-34B1 and complement to PBLs totally abrogates T-cell functions such as mitogen (PHA) stimulation and response in MLR, while addition of EDN-34B1 alone only affects MLR.
通过用人T细胞白血病细胞系HPB - ALL的细胞制备杂交瘤，获得了一种针对T细胞E花环受体的IgM单克隆抗体。该抗体名为EDN - 34B1，仅与T细胞系的细胞发生反应，包括三种T细胞白血病细胞系的细胞。它不与B细胞系的细胞、正常B细胞或单核细胞发生反应。EDN - 34B1识别一种分子量约为50,000的单一多肽，能够阻断E花环的形成，其与靶细胞的结合可被两种抗E受体抗体——单克隆抗体9.6和OKT - 11特异性阻断。EDN - 34B1对正常胸腺细胞和外周T细胞具有100%的细胞毒性，因此能够从混合淋巴细胞群体中常规去除此类细胞，并且即使免疫荧光研究表明两种抗体都与相同比例的外周血淋巴细胞（PBL）结合，但它杀死正常T淋巴细胞的比例比抗体9.6更高。这种现象可能归因于EDN - 34B1的IgM性质。将EDN - 34B1和补体添加到PBL中会完全消除T细胞功能，如丝裂原（PHA）刺激和混合淋巴细胞反应（MLR）中的反应，而单独添加EDN - 34B1仅影响MLR。
