Wang C Y, Azzo W, Al-Katib A, Chiorazzi N, Knowles D M
J Immunol. 1984 Aug;133(2):684-91.
We report in this paper the generation and characterization of three monoclonal antibodies, designated alpha BL1, alpha BL2, and alpha BL3, that recognize distinctive antigens unrelated to complement, Fc, and mouse erythrocyte rosette receptors, which are preferentially expressed on B lymphocytes. alpha BL1 recognizes a heat stable nonimmunoprecipitable antigen, possibly glycolipid in nature. Alpha BL2 recognizes a nonreducible single polypeptide with a m.w. of 68,000 that occasionally co-precipitates with a p29,34 complex of HLA-DR antigens. Alpha BL3 recognizes a nonreducible single polypeptide with a m.w. of 105,000 with an acidic pI point. We demonstrated that BL1 is expressed on fetal liver hematopoietic cells, a small subset (5 to 15%) of Ficoll-Hypaque-separated normal bone marrow cells, and on a subpopulation of nonadherent, non-E rosette-forming cells and granulocytes. BL2 is expressed on fetal liver hematopoietic cells, on 3 to 7% of normal bone marrow cells, and on a majority (40 to 70%) of nonadherent, non-E rosette-forming cells with a distinctive pattern similar to that of HLA-DR. BL3 is expressed on a subpopulation of nonadherent, non-E rosette-forming cells, and on occasional cells in the monocyte-enriched adherent cell population. The peak fluorescence for BL2 is substantially higher than that of BL1 and BL3, indicating higher BL2 antigen density. All three antigens are absent from thymocytes and E rosette-positive T cell fractions obtained from various lymphoid tissues. Cellular distribution of the BL antigens on various well-characterized established hematopoietic cell lines, leukemias, and malignant lymphomas, in conjunction with the results of the in vitro activation and TPA-induction experiments, suggest that BL1 is expressed during early developmental stages of B cell differentiation, whereas BL3 is expressed at the later stages. BL2 expression spans immature and mature stages of B cell differentiation, with the exception of mature plasma cells. The alpha BL antibodies described here should prove to be useful in the investigation of B cell differentiation and in the clinical diagnosis of lymphoid neoplasms.
我们在本文中报告了三种单克隆抗体的产生及特性，分别命名为αBL1、αBL2和αBL3，它们识别与补体、Fc及小鼠红细胞花环受体无关的独特抗原，这些抗原在B淋巴细胞上优先表达。αBL1识别一种热稳定的、不可免疫沉淀的抗原，其本质可能是糖脂。αBL2识别一种分子量为68,000的不可还原的单一多肽，它偶尔会与HLA - DR抗原的p29,34复合物共沉淀。αBL3识别一种分子量为105,000、具有酸性pI值的不可还原的单一多肽。我们证明，BL1在胎肝造血细胞、Ficoll - Hypaque分离的正常骨髓细胞的一小部分（5%至15%）以及非贴壁、非E花环形成细胞和粒细胞亚群上表达。BL2在胎肝造血细胞、3%至7%的正常骨髓细胞以及大多数（40%至70%）非贴壁、非E花环形成细胞上表达，其模式与HLA - DR独特模式相似。BL3在非贴壁、非E花环形成细胞亚群以及富含单核细胞的贴壁细胞群体中的偶尔细胞上表达。BL2的峰值荧光明显高于BL1和BL3，表明BL2抗原密度更高。从各种淋巴组织获得的胸腺细胞和E花环阳性T细胞组分中均不存在所有这三种抗原。BL抗原在各种特征明确的已建立造血细胞系、白血病和恶性淋巴瘤上的细胞分布，结合体外激活和TPA诱导实验结果表明，BL1在B细胞分化的早期发育阶段表达，而BL3在后期表达。BL2表达跨越B细胞分化的不成熟和成熟阶段，但成熟浆细胞除外。本文所述的αBL抗体应被证明在B细胞分化研究和淋巴瘤临床诊断中有用。
