Yang Can, Chen Jing, Zhou Huifen, Zeng Di, Wan Haitong, Yang Jiehong
School of Basic Medical Sciences, Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, China.
School of Life Sciences, Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, China.
Front Microbiol. 2024 Apr 29;15:1394304. doi: 10.3389/fmicb.2024.1394304. eCollection 2024.
In this study, we examined the therapeutic effects of Yinhuapinggan granules (YHPGs) in influenza-infected mice. We also examined how YHPGs affect the composition of the intestinal flora and associated metabolites.
We used the nasal drip method to administer the influenza A virus (IAV) H1N1 to ICR mice. Following successful model construction, the mice were injected with 0.9% sterile saline and low (5.5 g/kg), medium (11 g/kg), and high (22 g/kg) doses of YHPGs. The pathological changes in the lungs and intestines were evaluated by gavage for 5 consecutive days. Detection of sIgA, IL-6, TNF-α, INF-γ, and TGF-β cytokine levels in serum by enzyme-linked immunosorbent assay. Real-time fluorescence quantitative polymerase chain reaction and Western blot were used to measure the mRNA and protein expression of the tight junction proteins claudin-1, occludin, and zonula occludens-1 (ZO-1) in the colon. To assess the influence of YHPGs on the intestinal microbiota, feces were obtained from the mice for 16s rRNA sequencing, and short-chain fatty acids (SCFAs) were measured in the feces.
By reducing the production of pro-inflammatory cytokines and increasing the relative expression of claudin-1, occludin, and ZO-1 in colon tissues, YHPGs had a protective effect in tissues from the lungs and colon. When YHPGs were administered to mice with IAV infection, the relative abundance of , , , , , and increased, whereas the relative abundance of decreased.
The therapeutic mechanism of YHPGs against IAV infection in mice may be underpinned by modulation of the structural composition of colonic bacteria and regulation of SCFA production.
在本研究中，我们检测了银花平感颗粒（YHPGs）对流感感染小鼠的治疗效果。我们还研究了YHPGs如何影响肠道菌群的组成及相关代谢产物。
我们采用滴鼻法将甲型流感病毒（IAV）H1N1接种于ICR小鼠。成功构建模型后，给小鼠注射0.9%无菌生理盐水以及低（5.5 g/kg）、中（11 g/kg）、高（22 g/kg）剂量的YHPGs。连续5天通过灌胃评估肺和肠道的病理变化。采用酶联免疫吸附测定法检测血清中sIgA、IL-6、TNF-α、INF-γ和TGF-β细胞因子水平。运用实时荧光定量聚合酶链反应和蛋白质免疫印迹法检测结肠中紧密连接蛋白claudin-1、occludin和闭合蛋白1（ZO-1）的mRNA和蛋白表达。为评估YHPGs对肠道微生物群的影响，采集小鼠粪便进行16s rRNA测序，并测定粪便中的短链脂肪酸（SCFAs）。
通过减少促炎细胞因子的产生以及增加结肠组织中claudin-1、occludin和ZO-1的相对表达，YHPGs对肺和结肠组织具有保护作用。给感染IAV的小鼠施用YHPGs后， 、 、 、 、 和 的相对丰度增加，而 的相对丰度降低。
YHPGs对小鼠IAV感染的治疗机制可能是通过调节结肠细菌的结构组成和SCFA的产生来实现的。
